You have a 3 mL suspension of cells at a concentration of 480,000 cells per mL....
You have a 3 mL suspension of cells at a concentration of 480,000 cells per mL. How much medium (in mL) should you add to the suspension to adjust the concentration to 100,000 cells per mL?
Homework Answers
Answer: 11.4 ml of medium should be added to 3 ml of cell suspension.
Explanation: V1 C1 = V2 C2
V1 is the volume of cell suspension or stock and C2 is the concentration of the stock
V2 is the required volume and C2 is the required concentration.
We know V1 = 3 ml, C1 = 480000 cell/ml and C2 = 100000 cells/ml.
V2 = ?
V2 = (V1 x C1 ) / C2
= (3 x 480000) / 100000
= 14.4 ml will be the total volume of media with the cell suspension. so 14.4 - 3ml of cell suspension = 11.4 ml.
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