You have a tube with 8 ml of splenocytes at a concentration of 9.8 x 106...
You have a tube with 8 ml of splenocytes at a concentration of 9.8 x 106 cells/ml and anything else you may need. The diluting buffer is RPMI/2% FCS. You have at your disposal everything else you may need
Question: You need to make five (5) 96 well plates in which each well has 5 x 104 cells in a volume of 100ul. These are going to be used for a cytotoxicity assay. Describe how would you do this, showing your complete calculations and the final answer.
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You have a tube with 8 ml of splenocytes at a concentration of
9.8 x 106...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
You have a tube with 3 ml of lymphocytes that are at a concentration of 7...
You have a tube with 3 ml of lymphocytes that are at a concentration of 7 x 106 cells/ml. You also have a supply of cell culture media that can be used to dilute cells. You are asked to make up an 8 ml sample with a lymphocyte concentration of 3 x 105 cells/ml. How will you achieve this? Show your calculations and then write your answer.
It would be much appreciated if you can help me with the recipe of the buffer...
It would be much appreciated if you can help me with the recipe
of the buffer above. Thank you.
Cells are kept in suspension in RPMI, a media for growing cells. You have 30 mL of a suspension at a concentration of 7.2x10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1*10^5 cells when plated in the wells of a 96-well microtitre plate. How would you dilute...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
1a Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube...
1a Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. 1b Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of...
Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. Pipetting less than 1 μL is hard to do accurately. Do a dilution if less than 1μL After dilution,Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
1) The approximate cell yield of a T25 flask (for adherent cells) is 2.8 x 106...
1) The approximate cell yield of a T25 flask (for adherent cells) is 2.8 x 106 cells. The cell yield is the total amount of adherent cells at confluence. How many adherent cells would you expect to be in this flask at 80% confluence? 2) For adherent cells, the seeding density is usually between what range of % confluencies? 3) How many total cells would you place in a T75 flask with a cell yield of 8.4 x 106 cells...
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4 cells/mL and need to produce a solution of 56 cells/mL set up detailed serial dilution given 8mL of 4.1 x10^3 cells/ML and need approximitly 100 cells/mL. set up detailed serial dilution by determining the aliquot to be transfered (v1) from the solution to get 100 cells/mL in a final volume of 10 mL given 10mL cell suspension of NIH cells at 3.8 x10^5 cells/ML...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete...
1. You have been given a tube that contains 2.4 x 109
bacteria/ml. Diagram a complete dilution scheme so that you will
have a plate with 240 colonies on it.
How would you change the scheme you have drawn above so that the
plate had 100 colonies on it?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete dilution scheme so that you will have a plate with 240 colonies on it. (3...
Cells are kept in suspension in RPMI a media for glowing cells. You have 30 mL of a cell suspension at a concentration of 7.2 times 10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1 times 10^5 cells when plated in the wells of a 96-well microstate plate. How would you dilute some cells so you could prepare 48 such wells? Your answer should account for...
It would be much appreciated if you can help me with the recipe
of the buffer above. Thank you.
Cells are kept in suspension in RPMI, a media for growing cells. You have 30 mL of a suspension at a concentration of 7.2x10^6 cells/mL. You need to dilute a portion of these cells such that 100 mu L of the new suspension will deliver 1*10^5 cells when plated in the wells of a 96-well microtitre plate. How would you dilute...
1. You have been given a tube that contains 2.4 x 109
bacteria/ml. Diagram a complete dilution scheme so that you will
have a plate with 240 colonies on it.
How would you change the scheme you have drawn above so that the
plate had 100 colonies on it?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete dilution scheme so that you will have a plate with 240 colonies on it. (3...
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