Let-7 is a partially complementary miRNA that regulates the expression of lin-41 in C.elegans (worms). The...
Let-7 is a partially complementary miRNA that regulates the expression of lin-41 in C.elegans (worms). The let-7 sequence is deleted in mutant worms. In the mutant worms (compared to
normal worms), what amounts of the following would you expect to see?
a. Let-7 miRNA
b. Lin-41 mRNA
c. Lin-41 protein
(a. is less, b. is the same amount, and c. is more, but why?)
Homework Answers
When the gene for Let-7 miRNA is deleted in mutant worms, expression of Let-7 miRNA would be comparatively less in the mutant worms. Since Let-7 miRNA is responsible for regulating the expression of lin-41, in mutant worms, we can expect an increase in the expression of lin-41 protein. Because, when there is not enough let-7 miRNA to regulate, the expression of lin-41 would go unregulated. But since let-7 miRNA is not affecting the transcription of lin-41 gene, the concentration of lin-41 miRNA is expected to remain the same.
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Let-7 is a partially complementary miRNA that regulates the
expression of lin-41 in C.elegans (worms). The...
1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The...
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someone please help! i have a brain fart :/ tations and Regulation of Gene Expression 6....
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Page < 2 > of 2 D. The trp operon would be expressed at high levels,...
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1 2 3 4 5 lane lane (fig60) -- Lin-41 western blot let-7 northern blot The images above show a northern blot using a probe to identify the let-7 miRNA (left), and a western blot (right) with a primary antibody to recognize Lin-41, a let-7 target. Assume each lane in both the northern and western blots come from the same sample. Which lane best matches each scenario described listed below? Normally processed let-7 miRNA let-7 miRNA in a Drosha mutant...
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someone please help! i have a brain fart :/
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