Question

1. Do all parts: a. The sequence of a gene on mRNA is normally AUGCCCGACUUU. The point mutation in the gene results in the MRNA sequence AUGCCGGACUUU. What are the amino acid sequence for the normal and mutant proteins? Say, with an explanation whether you expect this to be a silent mutation. b. Why is DNA polymerase said to be template-directed? If a RNA had the nucleotide sequence 5'-AUGCCAUAACGAUACCCCAGUC-3', what was the sequence of the DNA strand that was transcribed and its complementary strand? C. d. What are the roles of DNA, RNA, and protein in information flow in biological systems? e. How is the polymerase chain reaction applied in forensic science?

Answer 1

a) amino acid sequence of normal protein and protein from mutant gene are the same thst is 'met pro asp phe'. This kind of mutation in which the difference in nucleotide sequence doesn't show any difference in the amino acid sequence of the protein are called as silent mutation's.

b)DNA polymerase replicate or synthesise new strand of DNA using nucleotides which are exactly complementary to the original strand which generally considered as template. Hence it is called as template dependent replication.

C)the DNA strand that was transcribed is TAC GGT AT GCT AT GGT CAG

the compementory strand is ATG CCA TAA CGA TAC CCA GUC.

d)the DNA contains genes which governs the all charectors of organisms, while RNA is the mediator between DNA and protein, on the other hand proteins works at the expression end of the dogma which either play structural role by working as building blocks or catalytic role in metabolism by working as enzymes

e) PCR can be used to identify guilty from accused by comparing samples collected at crime, it also helps to resolve parental disputes by comparing samples from children with possible parents.