Bacteria are grown in 15N (heavy) medium and then transferred to 14N (light) medium and are...
Bacteria are grown in 15N (heavy) medium and then transferred to 14N (light) medium and are allowed to replicate for 1 generation. The DNA is subsequently isolated and centrifuged in a CsCl2 gradient to yield what type of gradient band(s)?
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one intermediate band
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one heavy and one light band
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one heavy band
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one light band
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one heavy and one intermediate band
Homework Answers
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Bacteria are grown in 15N (heavy) medium and then transferred to
14N (light) medium and are...
Question 7 (1 point) Saved DNA replication occurs through the semi-conservative replication mechanism; what was observed...
Question 7 (1 point) Saved DNA replication occurs through the semi-conservative replication mechanism; what was observed in the Meselson and Stahl experiment, in which DNA purified from E. coli growing on 14N-medium was centrifuged in a density gradient tube, by the third generation of DNA replication? One band representing "light" DNA OTwo bands: a light density band and a faint hybrid density band Three bands: one each of light density, hybrid density, and high density bands One band representing "heavy"...
In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (^15...
In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (^15 N) and then transferred the bacteria to a medium containing^14 N ("lighter" nitrogen) and allowed some of the the bacteria to undergo one round of replication and some bacteria to undergo two rounds of replication. They then performed density gradient centrifugation and looked to see where the DNA settled in the test tube. In the diagram below, draw bands indicating the density of the...
i dont understand 46 at all Question 46 In the late 1950s, Meselson and Stahl grow...
i dont understand 46 at all
Question 46 In the late 1950s, Meselson and Stahl grow bacteria in a medium containing "heavy nitrogen (N) and then transferred them to a medium containing different amount of time for growth and cell division and then isolated DNA and subjected it to density gradient centrifugation 0/1 point . They allowed mo 14N DNA 14N 1SN hybrid DNA STICHE 15N DNA pod A. B. C. D. E. Which of the results in the figure...
áctica #8 - Estructura del Ácido Nucléico, Rep... Help Save & Exit Submit Light Half-heavy Heavy...
áctica #8 - Estructura del Ácido Nucléico, Rep... Help Save & Exit Submit Light Half-heavy Heavy McGraw-Hill Education ellook ferences Suppose that, as in the Meselson-Stahl experiment, E. coll is grown for many generations in heavy nitrogen (N) precursors, then shifted to light nitrogen (14N) precursors for 1 or 2 generations. Select the test tube that best corresponds to the positions that DNA would be expected to migrate in the following hypothetical situations. (Not all letters may be used, and...
10. Assume that a strain of E. coli is grown for many generations in SN, a...
10. Assume that a strain of E. coli is grown for many generations in SN, a heavy isotope of nitrogen (14N). Under this condition, assume that the DNA has a density of 1.723 gm/cm (with water being 1.00 gm/cm'). Assume that DNA containing only I'N has a density of 1.700. Bacteria from the SN culture were washed in buffer and transferred to 4N medium for one generation. DNA was extracted. a. What would the expected density of the extracted DNA?...
Please explain which one I missed, I got a 8/10. Might be the last question that...
Please explain which one I missed, I got a 8/10. Might be the
last question that I missed!
ament Quiz -U01-202020 Fund of Biology | The Meselson-Stahl experiment demonstrated that DNA replication produces two DNA molecules each composed of _? Select one a One Old stand of nucleotides and One new strand of nucleotides b. A varying number of strands of old and new nucleotides Two strands of old and new nucleotides mixed d. Two new strands of nucleotides e....
You start with a cell that is grown in normal media. Subsequently DNA is allowed to...
You start with a cell that is grown in normal media. Subsequently DNA is allowed to replicate in an environment with all necessary enzymes, dATP, dCTP, dGTP, and radioactively labeled dTTP (3H thymidine) for 1 cell cycle, (resulting in 2 cells) and then switched back to normal, nonradioactive medium. Cell are allowed to replicate once more, so you end up with 4 cells. How many of these 4 cells with have radioactive dTTP incorporated in their DNA? Select one: a....
Please need help answering question A the pages of background information are posted thanks Read page...
Please need help answering question A the pages of background
information are posted thanks
Read page 196-197 and figure 6.20. regarding Meselson and
Stahl’s experiment regarding DNA replication. And Answer the
following question
If you are using this radioactive technique in mouse cells,
what would happen in each phase of G1, S, G2, mitosis and meiosis
assuming that you are grown cells in 15N medium for many
generations and cells in G1are then switched to 14N medium?
G1
S
G2...
please help answering You start with a cell that is grown in normal media. Subsequently DNA...
please help answering You start with a cell that is grown in normal media. Subsequently DNA is allowed to replicate in an environment with all necessary enzymes, dATP, dCTP, dGTP, and radioactively labeled dTTP (3H thymidine) for 1 cell cycle, (resulting in 2 cells) and then switched back to normal, nonradioactive medium. Cell are allowed to replicate once more, so you end up with 4 cells. How many of these 4 cells with have radioactive dTTP incorporated in their DNA?...
Thank you!! Question 59 1 pts Under experimental conditions, cells grown in a medium containing thymine...
Thank you!!
Question 59 1 pts Under experimental conditions, cells grown in a medium containing thymine would incorporate thymine into their DNA. If cells grown for a number of generations in a medium containing radioactive thymine were removed from this medium and allowed to replicate once using thymine that was not radioactive, what percent of these cells would now be radioactive? 100% 50% 0% 25%
Question 7 (1 point) Saved DNA replication occurs through the semi-conservative replication mechanism; what was observed in the Meselson and Stahl experiment, in which DNA purified from E. coli growing on 14N-medium was centrifuged in a density gradient tube, by the third generation of DNA replication? One band representing "light" DNA OTwo bands: a light density band and a faint hybrid density band Three bands: one each of light density, hybrid density, and high density bands One band representing "heavy"...
In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (^15 N) and then transferred the bacteria to a medium containing^14 N ("lighter" nitrogen) and allowed some of the the bacteria to undergo one round of replication and some bacteria to undergo two rounds of replication. They then performed density gradient centrifugation and looked to see where the DNA settled in the test tube. In the diagram below, draw bands indicating the density of the...
i dont understand 46 at all
Question 46 In the late 1950s, Meselson and Stahl grow bacteria in a medium containing "heavy nitrogen (N) and then transferred them to a medium containing different amount of time for growth and cell division and then isolated DNA and subjected it to density gradient centrifugation 0/1 point . They allowed mo 14N DNA 14N 1SN hybrid DNA STICHE 15N DNA pod A. B. C. D. E. Which of the results in the figure...
áctica #8 - Estructura del Ácido Nucléico, Rep... Help Save & Exit Submit Light Half-heavy Heavy McGraw-Hill Education ellook ferences Suppose that, as in the Meselson-Stahl experiment, E. coll is grown for many generations in heavy nitrogen (N) precursors, then shifted to light nitrogen (14N) precursors for 1 or 2 generations. Select the test tube that best corresponds to the positions that DNA would be expected to migrate in the following hypothetical situations. (Not all letters may be used, and...
10. Assume that a strain of E. coli is grown for many generations in SN, a heavy isotope of nitrogen (14N). Under this condition, assume that the DNA has a density of 1.723 gm/cm (with water being 1.00 gm/cm'). Assume that DNA containing only I'N has a density of 1.700. Bacteria from the SN culture were washed in buffer and transferred to 4N medium for one generation. DNA was extracted. a. What would the expected density of the extracted DNA?...
Please explain which one I missed, I got a 8/10. Might be the
last question that I missed!
ament Quiz -U01-202020 Fund of Biology | The Meselson-Stahl experiment demonstrated that DNA replication produces two DNA molecules each composed of _? Select one a One Old stand of nucleotides and One new strand of nucleotides b. A varying number of strands of old and new nucleotides Two strands of old and new nucleotides mixed d. Two new strands of nucleotides e....
Please need help answering question A the pages of background
information are posted thanks
Read page 196-197 and figure 6.20. regarding Meselson and
Stahl’s experiment regarding DNA replication. And Answer the
following question
If you are using this radioactive technique in mouse cells,
what would happen in each phase of G1, S, G2, mitosis and meiosis
assuming that you are grown cells in 15N medium for many
generations and cells in G1are then switched to 14N medium?
G1
S
G2...
Thank you!!
Question 59 1 pts Under experimental conditions, cells grown in a medium containing thymine would incorporate thymine into their DNA. If cells grown for a number of generations in a medium containing radioactive thymine were removed from this medium and allowed to replicate once using thymine that was not radioactive, what percent of these cells would now be radioactive? 100% 50% 0% 25%
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