why did restriction enzymes eveolve and why do they not cut the DNA of all host...
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Restriction enzymes are a group of enzymes which can cut DNA at a particular sequence. The particular sequence is also known as restriction site. These enzymes has evolved as a defence mechanism of bacteria. These protect the bacteria from the bacteriophage. Bacteriophage acts by incorporating it's genome into the host bacteria cell and then multiplies within it.
These enzymes cut the DNA of bacteriophage and protect the bacteria from infection.
Host bacteria resist the action of restriction enzymes by the help of methylation of bases. This process requires some enzymes such as methylase. The base sequence is modified using the enzyme so that the restriction enzymes are unable to cleave the DNA of the host cell and thus provides protection from it.
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why
did restriction enzymes eveolve and why do they not cut the DNA of
all host...
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA...
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA at a specific nucleotide sequence D. Copy DNA 25. Which of the following is an example of a differentiated cell? A. Embryonic Stem Cell B. Hematopoeitic Stem Cell C. Adult Stem Cell D. Liver Cell 26. An example of a small, circular DNA molecule used as a vector to transfer foreign DNA to a host cell is a A. plasmid B prion C. liposome...
Question 18 4 pts What role do restriction enzymes play in bacteria? How do bacteria protect...
Question 18 4 pts What role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from the action of restriction enzymes? Change the surface proteins of bacteria; since DNA is not protein, there is no need for protection Cut foreign DNA into pieces; bacteria have RNA genomes. Destroy invading viral DNA: bacterial DNA does not contain the restriction enzyme recognition sequences. Restrict the growth rate of bacteria; bacterial DNA is restriction enzyme resistant. Question 18 4...
Why do restriction enzymes need to be kept on ice? What order should the DNA, enzyme,...
Why do restriction enzymes need to be kept on ice? What order should the DNA, enzyme, water and buffer be added to the microcentrifuge tube for a restriction digest? If lambda DNA is linear, how many times would the enzyme have to cut the DNA to generate five DNA fragments? Would a shorter DNA fragment move faster or slower through the agarose gel than a longer fragment? Why?
What are restriction enzymes and how do they affect DNA? Why do some fragments move quickly...
What are restriction enzymes and how do they affect DNA? Why do some fragments move quickly and some move slowly through an agarose gel? How can type II restriction enzymes and agarose gels be used to identify samples from individuals with the similar DNA sequence?
Which is not true about restriction enzymes? a. They cut RNA b. They evolved as a...
Which is not true about restriction enzymes? a. They cut RNA b. They evolved as a bacteria defense mechanism c. They can leave single-stranded overhanging sequences called Sticky ends d. They recognize a specific target sequence e.They cut DNA
Restriction Enzymes are naturally found in bacteria that use them to A. Replicate DNA B. Correct...
Restriction Enzymes are naturally found in bacteria that use them to A. Replicate DNA B. Correct mutated DNA C. Cut up the DNA of invading Bacteriophage D. complete conjugation
A restriction map lists the locations of DNA sequences that are cut by a particular restriction...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
find the errors Restriction enzymes recognize specific DNA sequences and cut each strand of DNA at...
find the errors
Restriction enzymes recognize specific DNA sequences and cut each strand of DNA at specific locations at the target sequence. The result of digesting a particular genome with a particular restriction enzyme is a collection of restriction fragments of defined length and composition. These can be used to generate restriction maps or create pieces with sticky ends. These sticky ends can be used to attach to other fragments that have sticky ends caused by cutting with a different...
Which of the following is not a use of restriction enzymes? A. Bacteria produce them to...
Which of the following is not a use of restriction enzymes? A. Bacteria produce them to cut up foreign viral DNA B. Making recombinant DNA C. Cutting a vector when cloning a gene D. Replicating DNA during PCR
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in specific sequences c. nuclear proteins that are involved in transcription d. components of the ribosomes involved in protein synthesis 15. The first step in southern blotting is a. converting DNA into RNA b. cutting high molecular weight DNA into smaller pieces c. converting RNA into DNA d. radioactively labeling the DNA so it can be detected after the procedure is complete 16. The major...
24. What do restriction enzymes do? A Randomly cut DNA B. Produce protein C. Cut DNA at a specific nucleotide sequence D. Copy DNA 25. Which of the following is an example of a differentiated cell? A. Embryonic Stem Cell B. Hematopoeitic Stem Cell C. Adult Stem Cell D. Liver Cell 26. An example of a small, circular DNA molecule used as a vector to transfer foreign DNA to a host cell is a A. plasmid B prion C. liposome...
Question 18 4 pts What role do restriction enzymes play in bacteria? How do bacteria protect their own DNA from the action of restriction enzymes? Change the surface proteins of bacteria; since DNA is not protein, there is no need for protection Cut foreign DNA into pieces; bacteria have RNA genomes. Destroy invading viral DNA: bacterial DNA does not contain the restriction enzyme recognition sequences. Restrict the growth rate of bacteria; bacterial DNA is restriction enzyme resistant. Question 18 4...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
find the errors
Restriction enzymes recognize specific DNA sequences and cut each strand of DNA at specific locations at the target sequence. The result of digesting a particular genome with a particular restriction enzyme is a collection of restriction fragments of defined length and composition. These can be used to generate restriction maps or create pieces with sticky ends. These sticky ends can be used to attach to other fragments that have sticky ends caused by cutting with a different...
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