What might be wrong with the 1X TAE for an agarose gel to max out the...
- What might be wrong with the 1X TAE for an agarose gel to max out the amperage (I) and result in a lower voltage (v). (pH 8.3)
Homework Answers
Th ions which are provided by TAE buffer were not sufficient to carry out the electricity for electrolysis and the components are not functioning properly like EDTA to chelate divalent ions as a result the ions are not enough in the Gel for proper electrolysis process and thus amperage and voltage difference decreases resulting in failure of separation of DNA.
So , here 2× or more TAE must be used.
Please hit like.
Add Answer to:
What might be wrong with the 1X TAE for an agarose gel to max
out the...
You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red....
You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red. For a total gel volume of 75ml, calculate the mass of agarose powder and volume of Gel Red concentrate needed. Show your work to be eligible for partial credit. Info about stocks: Agarose stock is powder Gel Red concentrate is 25,000X liquid
Can anyone show me step-by-step on how to do the agarose calculations? This week we will...
Can
anyone show me step-by-step on how to do the agarose calculations?
This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...
Petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml...
9,10,12
petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
can you please answer all three questions. Thank you so much!! QUESTION 3: What is the...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
After PCR is performed the products are run out on an agarose gel. In the figure...
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
FULL image displayed under of the expected vs actual results of agarose gel analysis (the top...
FULL image displayed under of the expected vs actual results of
agarose gel analysis (the top picture is expected and bottom is the
result)
Question: You conclude that the error occurred while preparing
arose gel. What mistake was made? Why does it look like the picture
above and not how you expected?
Gel Question #1: During Lab 7, you and your three teammates performed gel electrophoresis as described in the lab manual. Your team was especially careful while carrying out...
The PCR procedure can be performed with fluorescently labeled primers and polyacrylamide gel electrophoresis (PAGE). What...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
is a nucleus the sole source of DNA in a eukaryotic cell? WORKSHEET: EXERCISE 10 NAME:...
is
a nucleus the sole source of DNA in a eukaryotic cell?
WORKSHEET: EXERCISE 10 NAME: DATE: SECTION Twenty microliters (20 ㎕) of each sample was loaded into your gel. Convert 2OuL to milliliters (mL). I. 2. Describe the charge on the DNA molecule and specify which component of the molecule is responsible for contributing to that charge. Why do some DNA samples travel further in the gel than others? 3. 4. Once electrophoresed, how are the DNA bands visualized?...
This a question I am asking purely out of curiosity, no right or wrong answers, I...
This a question I am asking purely out of curiosity, no right or wrong answers, I just want to hear your opinion. What are some real life applications or examples of electric potential or Coulomb's Law? For example, I know that DNA is negatively charged and is attracted to positively charged poles during gel electrophoresis. I am mostly interested in finding out what other things in life utilize postive/negative charge relationships. Doesn't have to be fancy, any examples are fine...
Not sure how to figure out what formulas to use here esolving gel with DI water,...
Not sure how to figure out what formulas to use here
esolving gel with DI water, and blot dry with a paper towel. (demo) a small flask (clean and reuse the old flask), mix com , swirl to mix and add the mixture (with a Pasteur pipet) to the gel ur ponents of the s dly in sert the comb. Some acrylamide may spill out of the gel. Repeat erize at room temperature for 45 min. below should be drawn,...
Can
anyone show me step-by-step on how to do the agarose calculations?
This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...
9,10,12
petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
can you please answer all three questions. Thank you so
much!!
QUESTION 3: What is the purpose of the Tris-Acetate-EDTA (TAE) buffer that the agarose gel is prepared with and submerged in for running? What would happen if you used water to prepare and run the gel instead of TAE buffer? (You should conduct an internet search to answer this question) [2 marks] QUESTION 1: Several factors (including agarose gel concentration, time and current) affect migration of DNA fragments through...
After PCR is performed the products are run out on an agarose
gel. In the figure below, grey bands represent the wells the PCR
product was loaded into. The white bands represent DNA fragments
produced by PCR. The target fragment amplified by the primers was
1,500 bp in size.
The ladder is a standard DNA ladder containing bands of various
sizes between 5,000 and 1,000 bp. The negative control contained
only molecular grade water*. The positive control contained DNA
known...
FULL image displayed under of the expected vs actual results of
agarose gel analysis (the top picture is expected and bottom is the
result)
Question: You conclude that the error occurred while preparing
arose gel. What mistake was made? Why does it look like the picture
above and not how you expected?
Gel Question #1: During Lab 7, you and your three teammates performed gel electrophoresis as described in the lab manual. Your team was especially careful while carrying out...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
is
a nucleus the sole source of DNA in a eukaryotic cell?
WORKSHEET: EXERCISE 10 NAME: DATE: SECTION Twenty microliters (20 ㎕) of each sample was loaded into your gel. Convert 2OuL to milliliters (mL). I. 2. Describe the charge on the DNA molecule and specify which component of the molecule is responsible for contributing to that charge. Why do some DNA samples travel further in the gel than others? 3. 4. Once electrophoresed, how are the DNA bands visualized?...
Not sure how to figure out what formulas to use here
esolving gel with DI water, and blot dry with a paper towel. (demo) a small flask (clean and reuse the old flask), mix com , swirl to mix and add the mixture (with a Pasteur pipet) to the gel ur ponents of the s dly in sert the comb. Some acrylamide may spill out of the gel. Repeat erize at room temperature for 45 min. below should be drawn,...
Most questions answered within 3 hours.
-
A space vehicle is coasting at a constant velocity of 18.1 m/s
in the +y direction...
asked 55 minutes ago -
5) Use the following balanced equation to answer questions
(a)-(i).
3 Cu(s) + 8 HNO3(aq) →...
asked 37 minutes ago -
A three-year maturity bond with a 12% annual coupon rate is
currently traded at par $1000...
asked 46 minutes ago -
1.
What is the minimum volume to use in the Spec 20 cuvette in order
to...
asked 1 hour ago -
A builder wants to install wall-to-wall carpeting in a living
room of length 8.87 m and...
asked 1 hour ago -
Organizational change can produce anxiety in those affected by
the change. In what ways can change...
asked 1 hour ago -
Ozone in the atmosphere may react with nitric oxide.
From the following data, calculate the DG...
asked 1 hour ago -
Steven Long, a bond analyst, is analyzing a convertible bond.
The characteristics of the bond are...
asked 1 hour ago -
Michael Bordellet is
the owner/pilot of Stellar Air Service. The company flies a daily
round trip...
asked 1 hour ago -
Hi, I need to change the conversion where stack is into a class
stack and add...
asked 1 hour ago -
Consider the log logistic CDF F(t)=1/(1+(t/α)-β)
Find f(t), S(t), h(t), and H(t).
It can be shown...
asked 1 hour ago -
A gaseous mixture of methane and water vapor at
atmospheric pressure and 500 ° C is...
asked 1 hour ago