Why are there multiple results returned when you input a sequence into BLAT or BLAST? How do you know which is most relevant? Does that make the others irrelevant ?
BLAST = Basic Local Alignment Search Tool
It is a software tool used to search unknown sequences using
sequence similarity/identity.
When a BLAST search is performed, several results are
obtained.
These results represent multiple sequences from different organisms
that exhibit sequence similarity with the query sequence.
If the query sequence is conserved, the number of results will be
high.
Among the BLAST results, we have to choose the result which has maximum similarity/identity and sequence coverage.
It does not make other results irrelevant. It just shows that such sequences are also found in other organisms.
Why are there multiple results returned when you input a sequence into BLAT or BLAST? How...
Use BLAST to find DNA sequences in databases Perform a BLAST search as follows: Do an Internet search for “ncbi blast”. Click on the link for the result: BLAST: Basic Local Alignment Search Tool. Under the heading “Basic BLAST,” click on “nucleotide blast”. pMCT118_F 5’- GAAACTGGCCTCCAAACACTGCCCGCCG -3’ (forward primer) pMCT118_R 5’- GTCTTGTTGGAGATGCACGTGCCCCTTGC -3’ (reverse primer) Enter the pMCT118 primer (query) into the search window. (see Moodle metacourse page for the file – just copy and paste the sequence into the...
Why and when to use multiple? How to assess whether the valuation results make sense in terms of the EV/EBIDTA multiple.
please answer questions with the following BLAST results For your GenBank assignment due at 12 noon on Thursday May 23rd (don't forget, I will not accept late papers): 1. Dr. Monsen-Collar will tell you the number of a sequence posted on Canvas. Copy the entire sequence and use it in a BLAST search to determine what gene the sequence is. Answer the following questions: A. What gene is your unknown sequence likely to be? How do you know it's a...
Discussion W1 - Multiple Intelligences Watch Howard Gardner's video on Multiple Intelligences and answer the following questions: 1. How is the concept of multiple intelligences relevant to the world of business? How is it relevant to leading others? 2. Which type of intelligence that Gardner speaks to do you feel is most important in the business world to have, and why?
Note that the sequence a(n) = Crn is a constant multiple of the well-known geometric sequence you studied in Calculus II. We know that the behaviour of this sequence depends on both r and the sign of C. Read the relevant section in your Calculus text to create a table which discusses the different types long-term behavior, limn→∞ a(n), you observe. Question: Create a table which discusses the different types long-term behavior, limn→∞ a(n), you observe.
-how do you not know you are not activating multiple sub population when you are activating one subpopulation. what experiments would you do to verify this.
Can you explain how do you know when to add probabilities or multiple probabilities?
In detail, please explain how to do D and E 15.1 The results of a multiple regression based on nine observations are shown in Table 15.11. Based on these results answer the following questions: a. What percentage of variance is explained by the regression? TABLE 15.11 Results of a Multiple Regression Analysis 1.3 2.7 0.5 5.0 3.6 1.8 0.6 8.3 Intercept 75.3 Coefficient of multiple correlation 0.95 Standard deviation of errors 12.0 F-value 14. b. Is the regression significant at...
In looking through sequence information from the first assignment, you may have noticed there was no ONE TRUE SEQUENCE, and that there were quite a few ways to present the information with different databases and multiple entries for each database. Of most importance to us right now is understanding why it matters if the sequence being described is genomic or if it was an mRNA - and if it was an mRNA – was it a splice-site variant. A) What’s...
How can you tell which of the two following equations to use when solving problems and why do the two different equations exist? Why does one have moles and the other mass? How does this affect how we find internal energy for instance? I know that U= Q- W but wouldn't know which Q to use. CynAT specific mass change in heat added heat temperature