1. When performing a Gram stain on an unknown sample, what is the best way to...
1. When performing a Gram stain on an unknown sample, what is the best way to know if pink cells are truly Gram negative? (i.e., how will you know if you did the procedure correctly?)
Homework Answers
Gram-positive bacteria have a thick mess-like cell wall made of peptidoglycan and as a result are stained purple by crystal violet, whereas gram-negative bacteria bacteria have a thinner layer, so do not retain the purple stain and are counter-stained pink by safranin.
Add Answer to:
1. When performing a Gram stain on an unknown
sample, what is the best way to...
A) You perform a gram stain on an unknown and two controls. The gram positive control...
A) You perform a gram stain on an unknown and two controls. The gram positive control organism appears pink when you observe it microscopically. Your unknown organism also appears pink. Is your unknown organism gram positive, gram negative, or can't tell? B) what is the reason you could have the above result?
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
A Gram stain of unknown bacterium A grown for 24 hours show purple cells. Is bacterium...
A Gram stain of unknown bacterium A grown for 24 hours show purple cells. Is bacterium A Gram positive or Gram negative? A gram stain of bacterium A grown for 72 hours shows both purple and pink stained cells. Additional analysis shows that bacterium A is NOT contaminated. Explain these results. You are given 2 nutrient broth tubes each containing a pure culture of one of two Gram positive bacteria. Unfortunately, the labels have washed off and you are asked...
Microbiology Q1 Q2 Q3 Bonus (+1 each) Select all of the responses that indicate the importance...
Microbiology
Q1
Q2
Q3
Bonus (+1 each) Select all of the responses that indicate the importance of the Gram stain. Gram stain results can help classify bacteria. Gram stain results can help classify yeast. Gram stain results can assist in diagnosing bacterial infections. Gram stain results can assist in choosing an appropriate drug treatment. Gram stain results are no longer useful in medicine. Bonus (+1) What role does crystal violet play in the Gram stain procedure? primary stain secondary stain...
What is the structure and arrangement for the following unknown bacteria? What is the Gram Stain?...
What is the structure and arrangement for the following unknown bacteria? What is the Gram Stain? Select one: a. Gram negative Streptobacilli b. Gram positive Streptobacilli c. Gram positive Staphylbacilli d. Gram positive Staphylcocci
5. You use the Gram stain to try to differentiate bacteria in a sample. You find...
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
Help with 1,3 4,5and 7 please God bless u morphology morphologies Ex. 5 Questions 1. Gram-positive...
Help with 1,3
4,5and
7 please God bless u
morphology morphologies Ex. 5 Questions 1. Gram-positive cultures that are older than 24hrs can stain as Gram negative sometimes. Why does this happen? 2. Why is iodine important in the Gram stain? 3. Suppose you do a Gram stain on a pure culture of Gram-positive cocci. When you view the What should slide, you see some purple spheres and red spheres adjacent to one another. you conclude and why? 으..@ 흐...
When performing the gram stain, the the dye safranin is suspended in a 95% ethanol solution....
When performing the gram stain, the the dye safranin is suspended in a 95% ethanol solution. If only 96% ethanol is available, how many mL of ethanol should be added to achieve a final volume of 25 with the correct percent ethanol? Report your answer to 2 decimal places. Perform all calculations in a single step using Excel to avoid accumulated rounding error.
Questions 1 Often, upon doing a negative pom doing a negative stain of unencapsulated Gram-negative cells,...
Questions 1 Often, upon doing a negative pom doing a negative stain of unencapsulated Gram-negative cells, a small halo will be seen around the cells. Why is this the case? What external structure of Gram-negative cell will acude the negative stain? 2 What are the functions of a bacterial capsule? 3 Does a species produce a capsule under all conditions of cultivation? Explain. 4 Why is the size of a bacterium more accurate for a negative stain than a direct...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
A Gram stain of unknown bacterium A grown for 24 hours show purple cells. Is bacterium A Gram positive or Gram negative? A gram stain of bacterium A grown for 72 hours shows both purple and pink stained cells. Additional analysis shows that bacterium A is NOT contaminated. Explain these results. You are given 2 nutrient broth tubes each containing a pure culture of one of two Gram positive bacteria. Unfortunately, the labels have washed off and you are asked...
Microbiology
Q1
Q2
Q3
Bonus (+1 each) Select all of the responses that indicate the importance of the Gram stain. Gram stain results can help classify bacteria. Gram stain results can help classify yeast. Gram stain results can assist in diagnosing bacterial infections. Gram stain results can assist in choosing an appropriate drug treatment. Gram stain results are no longer useful in medicine. Bonus (+1) What role does crystal violet play in the Gram stain procedure? primary stain secondary stain...
What is the structure and arrangement for the following unknown bacteria? What is the Gram Stain? Select one: a. Gram negative Streptobacilli b. Gram positive Streptobacilli c. Gram positive Staphylbacilli d. Gram positive Staphylcocci
5. You use the Gram stain to try to differentiate bacteria in a sample. You find that all of the cells are Gram negative, but the teaching assistant insists there are both Gram positive and Gram negative cells present in the sample you are given. You definitely smeared a loop of the sample onto the slide and your fingers are stained a little purple and also a little red from handling the sides of the slide. Give a reason why...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
Help with 1,3
4,5and
7 please God bless u
morphology morphologies Ex. 5 Questions 1. Gram-positive cultures that are older than 24hrs can stain as Gram negative sometimes. Why does this happen? 2. Why is iodine important in the Gram stain? 3. Suppose you do a Gram stain on a pure culture of Gram-positive cocci. When you view the What should slide, you see some purple spheres and red spheres adjacent to one another. you conclude and why? 으..@ 흐...
When performing the gram stain, the the dye safranin is suspended in a 95% ethanol solution. If only 96% ethanol is available, how many mL of ethanol should be added to achieve a final volume of 25 with the correct percent ethanol? Report your answer to 2 decimal places. Perform all calculations in a single step using Excel to avoid accumulated rounding error.
Questions 1 Often, upon doing a negative pom doing a negative stain of unencapsulated Gram-negative cells, a small halo will be seen around the cells. Why is this the case? What external structure of Gram-negative cell will acude the negative stain? 2 What are the functions of a bacterial capsule? 3 Does a species produce a capsule under all conditions of cultivation? Explain. 4 Why is the size of a bacterium more accurate for a negative stain than a direct...
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