Question

1. An experimental multiplex PCR examines the flagellin genes expressed by several strains of Salmonella enterica Typhimurium; Type X, Y, or Z.

(a) You design two sets of primers per flagellin type you want to amplify. The following primers were designed for Type X. Assuming the bases were appropriate to the DNA region of interest, would (either / both / none) of the primer sets be appropriate to your test? Explain your choice in less than 30 words.

Primer set 1 a. 5’CCATAACGT b. 5’GTTATGGTG

Primer set 2 c. 5’AACGTCCAT d. 5’GCTCAAGCT

Answer 1

Only one primer set can be utilized at a time for a test either primer set 1 or primer set 2. Both of these sets cannot be utilized at same time for test because the base pairs arranged in these primer sets are in different sequence that means that triplet of base pairs forms one codon and code for a particular amino acid and as they are in different order they will code for different proteins in different primer set.