You have5 mL ofa concentrated stock solution of the vitamin biotin whose concentration is 10 mg/mL....
You have5 mL ofa concentrated stock solution of the vitamin biotin whose concentration is 10 mg/mL. You will be making 10 mL of nutrient medium and want the final biotin concentration of the medium to be 50 μg/mL. How will you formulate this nutrient medium? HINT: Don’t forget to convert your biotin concentrations to the same units, and use a multi-step dilution.
Homework Answers
Given-
50 ug/ ml = 0.05 mg/ml
So for total 10 ml we need 0.5 mg.
Stock solution has 10 mg/ml = 10mg/1000 uL.
Or 0.5 mg/50 microliter. ( only 0.5 mg we need for 10 ml).
So we will take 50 microliter of stock solution and will add rest of the water to make the 50 ug/ml of biotin solution.
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