Assume that you have a stock solution of epinephrine at a concentration of 3 mg/mL. Knowing that the pipette you will use delivers 20 drops/mL, calculate the number of drops of the stock solution that must be added to a smooth muscle bath containing 27 mL of Locke’s solution so that the final concentration of epinephrine in the muscle bath will be 173 µg/mL.
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Assume that you have a stock solution of epinephrine at a concentration of 3 mg/mL. Knowing...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
1) A stock solution has a concentration of 100 µg/mL. If 20 µL of stock is added to a beaker. What is the mass of the dye in µg? 2) If the absorbance of the same dye (after diluted with water to 3 mL) is 0.3 AU, what is A/µg of the dye used?
Stock glucose (C6H12O6) solution with concentration 50.0 mmol/L. 0.30 mL of such stock solution and added water to each vial to complete 10.0 ml. Final glucose concentrations in mg/dL?
You have5 mL ofa concentrated stock solution of the vitamin biotin whose concentration is 10 mg/mL. You will be making 10 mL of nutrient medium and want the final biotin concentration of the medium to be 50 μg/mL. How will you formulate this nutrient medium? HINT: Don’t forget to convert your biotin concentrations to the same units, and use a multi-step dilution.
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
You have a stock solution of 25 mg/mL igG and want to make 2000 microliters of protein standard at a concentration of 1.5 mg/mL. What volume of igG stock would you need?
3. Concentrations in solids and solution. You send a 10-g sample of rice to the lab for the determination of the total arsenic content. The analyst dissolves 1.0 g of the rice in 5.0 mL of concentrated acid and then dilutes the solution to a final volume of 25.0 mL. When analyzed by ICP-MS, the solution concentration is found to be 35 µg L-1. What is the concentration of arsenic in the rice (in µg kg-1)? ___________ ppb Hint: Follow...
You have a stock solution of enzyme (125 kD) at a concentration of 1mg/ml. You want to achieve a final enzyme concentration of 36nM in a 1ml reaction volume. What volume of the enzyme stock solution will you add to the reaction?