You have a stock solution of 25 mg/mL igG and want to make 2000 microliters of...
You have a stock solution of 25 mg/mL igG and want to make 2000 microliters of protein standard at a concentration of 1.5 mg/mL. What volume of igG stock would you need?
Homework Answers
Vol of stock required = desired vol wanted × final concentration / stock concentration
Final volume = 2000 ul = 2 ml
Vol = 2 × 1.5 / 25 = 0.12 ml = 120 ul
Add Answer to:
You have a stock solution of 25 mg/mL igG and want to make 2000
microliters of...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
(17) Suppose you have stock solution with a solute concentration of 10 mm. If you wanted...
(17) Suppose you have stock solution with a solute concentration of 10 mm. If you wanted to dilute this solution to make a total of 1 ml of a 3 mM solution, what volume of stock and what volume of water would you use? Give your answer in microliters (ul), and show your calculations. Volume of stock3 ul Volume of water · T ul
You have a stock solution of enzyme (125 kD) at a concentration of 1mg/ml. You want...
You have a stock solution of enzyme (125 kD) at a concentration of 1mg/ml. You want to achieve a final enzyme concentration of 36nM in a 1ml reaction volume. What volume of the enzyme stock solution will you add to the reaction?
You have an enzyme solution at a concentration of 5mg/ml. The enzyme activity you determine is...
You have an enzyme solution at a concentration of 5mg/ml. The enzyme activity you determine is 0.1 Units per microliters of undiluted protein. What is the specific activity of the enzyme (U/mg)?
You have a 1 M stock solution of TBE buffer. You want to make seven 500...
You have a 1 M stock solution of TBE buffer. You want to make seven 500 ml bottles of 0.25 M TBE buffer. What is the minimum amount of 1 M TBE stock solution that you would need to make all seven bottles? (ml)
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
(17) Suppose you have stock solution with a solute concentration of 10 mm. If you wanted to dilute this solution to make a total of 1 ml of a 3 mM solution, what volume of stock and what volume of water would you use? Give your answer in microliters (ul), and show your calculations. Volume of stock3 ul Volume of water · T ul
Most questions answered within 3 hours.
-
How much must I invest today if I want to withdraw 1/3 of my
original investment...
asked 26 minutes ago -
What is meant by a “term premium”?
What can explain such a premium? Is it a...
asked 2 hours ago -
23. Which molecule has only covalent bonds? A. CO2 B. Al2O3 C.
Mg3N2
24. The octet...
asked 2 hours ago -
the
total pressure for a mixture of N2O4 and NO2 is 2.20 atm. If Kp=
7.10...
asked 2 hours ago -
Delaware Corp. prepared a master budget that included $22,385
for direct materials, $28,600 for direct labor,...
asked 2 hours ago -
For pesticide HCB (hexachlorobenzene), its logarithmic partition
coefficient log Kow is 5.6. What would be the...
asked 2 hours ago -
A chemist requires 0.422 mil Na2CO3 for a reaction. How many
grams does this correspond to?
asked 2 hours ago -
If the vapor pressure of carvone is approximately 9.4 torr at 99
°C, approximately how much...
asked 3 hours ago -
A juice bottling company is carrying out a test at 10%
significance that the mean population...
asked 3 hours ago -
What is the reflectivity (in percent) needed to keep water at a
temperature near its freezing...
asked 3 hours ago -
Suppose that the average surface temperature of Earth is 300K.
Assume this is uniform over its...
asked 3 hours ago -
Draw the condensed structural formula for alkenes and alkynes or
the line-angle structural formula for cycloalkenes...
asked 3 hours ago