During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer...
During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 500ml of the A150 buffer?
Homework Answers
Given data,
Volume = 1 L
pH = 8.8
Molarity = number of moles
As it is 1 L, => ( 0.03 moles / 1.5 moles)
=> 0.02 L
=> 20 mL of Tris
So, take 20 mL of Tris
( 0.150 moles / 5 moles) = 0.03 L
= 30 mL of NaCl
So, take 20 mL of NaCl
Add Answer to:
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