Question

Q4)

A) Write the complement (also known as inverse complement) of the sequence below
indicating its directionality-5’-ACGGGCATTAGACATCCACGGAAATTCGCAG

B)Mark the recognition site(s) in this sequence for the restriction enzyme FokI which
recognizes the sequence GGATG

Answer 1

Ans 4)

A) Given sequence :    5'-ACGGGCATTAGACATCCACGGAAATTCGCAG-3'

complement sequence : 3'-TGCCCGTAATCTGTAGGTGCCTTTAAGCGTC-5'

B) Recognition site of Fokl is GGATG, so , recognition sites in the complement sequence would be:   

   3'-TGCCCGTAATCTGTAGGTGCCTTTAAGCGTC-5'

Answer 2

A) The complement of a DNA sequence is formed by replacing each base with its complementary base. The complementary bases are as follows: A (adenine) pairs with T (thymine), and C (cytosine) pairs with G (guanine). Additionally, the directionality of the sequence is indicated by the 5' and 3' ends.

Given sequence: 5’- ACGGGCATTAGACATCCACGGAAATTCGCAG -3’

Complement: 3’- TGCCCGTAATCTGTAGGTGCCGTTTAAGCGT -5’

B) The recognition site for the restriction enzyme FokI is "GGATG". To find this site in the given sequence, we need to look for the occurrence of "GGATG".

The sequence contains the recognition site "GGATG" at position 18 to 22 (reading from the 5' end). The sequence "GGATG" is located between the bases C and A in the given sequence.

So, the recognition site for the restriction enzyme FokI in the given sequence is:

5'- ACGGGCATTAGACATCCAGGATGGAAATTCGCAG -3'