Question

Part B. Please CHOOSE FOUR of the following FIVE questions, indicating clearly which you want to be graded. Do not answer all of them!! (15 points each.) B1. Please consider the restriction enzymes Apal and PspOMI (OMG, who THINKS of these names???). Anyway, these enzymes have the following recognition/cut sequences, with the * indicating the cut site: Apali 5'G GGCC*C 3 3'C *C C G G G 51 PspOMI: 5' G *GG CCC 31 3' C C C G G# G 57 For each of these, please state whether the overhangs are s'ends or 3'ends. Another enzyme, Eagl, cuts at the sequence: 5' C# G GCC G 3' 3' G C C G G* C 5' Can a molecule that has been cut with Eagl be annealed for one that is cut with Apal. PSRMOL neither, or both? Please demonstrate or explain verbally,

Answer 1

The restriction enzymes cut the DNA molecules at specific sites and produces 5' overhanging strand and 3' overhanging strand which can get annealed.

But in these DNA sequences they all could not get annealed with Eag1 because these overhangs ends are not complementary to eachother.