Question

Please answer number 21-25, explain and clearly indicate which number you are answering. Thanks in advance!

(Q21-25) We have the following double-stranded DNA sequences, which codes for a 10 amino acid long protein gene. Use the codon table and answer the questions.

5'-CCTGTGTCACTCACAGGGGATGGTATCACAGTGAGTCATGGGTTT-3'

3'-GGACACAGTGAGTGTCCCCTACCATAGTGTCACTCAGTACCCAAA-5'

Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Second letter UUU pne UCU UAU UAC/ UCC UUC UUA Ser UGU Tyr UGC UAA Stop UGA Stop UAG Stop UGG Trp UCA Leu UUG UCG CCU) CAU His CGU CGC CCC CAC CUU) CUC Leu CUA CUG) > Pro CCA CAA CGA CAG GIn CGG First letter ODOC DOC DOC DOC Third letter AAU) AGU AUU AUC lle AUAJ AUG Met ACU ACC ACA ACG) > Thr YA AAC AAA AGC AGA AGG AAG ys GCU) GCC GGU GUU GUC GUA GUG GAU ORO Asp GAC GAA Val GCA Ala GGC GGA GGG) GCG) GAG Glu

21. Which strand codes for this protein?

A. top

B. bottom

C. both strands

D. none of the above

22. If this is a eukaryotic gene, which RNA polymerase will make mRNA?

A. RNA Pol I

B. RNA Pol II

C. RNA Pol III

D. Primase

E. DNA polymerase

23. In which direction does this RNA polymerase move to make this RNA transcript?

A. from left to right

B. from right to left

C. in both directions

D. none of the above

24. Which is the right anticodon for the 2nd amino acid?

A. 5'-AGU-3'

B. 3'-AGU-5'

C. 5'-ACU-3'

D. 3'-ACU-3'

E. none of the above

25. Which is the 3rd amino acid for this protein?

A. Gln

B. His

C. Tyr

D. Arg

E. Pro