if you transfer 0.1 ml of a sample into a 99.9ml saline blank, what is the...

Question

Question

if you transfer 0.1 ml of a sample into a 99.9ml saline blank, what is the dilution?

Answer 1

Answer #1

For finding dilution ratio, simple equation can be given as

=ml of sample/(ml of sample+ml of diluent)

=(0.1/(0.1+99.9))

=0.1/100

=1/1000

So, this result gives us dilution, which is 1/1000

Answer 2

Similar Homework Help Questions

A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to...

A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to a bottle containing 99ml of sterile water. You then take 0.1 ml from this dilution bottle and transfer to a second bottle with 0.9 ml of sterile water. Finally, you transfer 1 ml to a Petri dish with nutrient agar. How many colonies would you expect to find on this plate? Would it be countable?
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...

Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
If you transfer 1.0 ml from Sample A into 99ml of water, how would you label...

If you transfer 1.0 ml from Sample A into 99ml of water, how would you label the water blank?

Seven Tubes Contain 1 ml of Saline. You add 1 ml of a 64 mg/ml sample...

Seven Tubes Contain 1 ml of Saline. You add 1 ml of a 64 mg/ml sample in tube 1. From tube 1 you take 1 ml and add it to tube 2. From Tube 2 you take 1 ml and add it to tube 3. You repeat this through tube 7. Write the sample concentration in each tube?
A sample of pond sediment was diluted by placing a 0.1 g into 9.9 ml of...

A sample of pond sediment was diluted by placing a 0.1 g into 9.9 ml of diluent, and 0.1 ml of this dilution was spread to two Nutrient Agar (NA) plates. After 3 d incubation, 186 colonies were counted on one plate and 198 on the other. What is the CFU/g of the sediment? (Assume that the density of the sediment is about that of water)
You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24...

You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 223 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-7)...

Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you...

Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would add to your sample to accomplish that? A. 10 ml B. 9 ml C. 9.9 ml D. 0.9 ml E. 0.99 ml 2. You have 0.5 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would...
Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you...

Microbiology Labs Practice Problems 1. You have 1 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would add to your sample to accomplish that? A. 10 ml B. 9 ml C. 9.9 ml D. 0.9 ml E. 0.99 ml 2. You have 0.5 ml sample of a patient's serum and you need to make a 1:10 dilutions of it in saline. How much saline you would...
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24...

You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8)...

Question 2 5 pts Transfer 0.1 mL of the bacterial suspension to the tube marked A1...

Question 2 5 pts Transfer 0.1 mL of the bacterial suspension to the tube marked A1 (containing 0.9 mL of 0.1M MgSO4). Transfer 0.01 mL (10 PL) of the bacterial suspension from A1 to the tube marked A2 (containing 0.99 mL of 0.1M MgSO4). Transfer 0.01 mL of the bacterial suspension from A2 into the microcentrifuge tube marked A3 (containing 0.99 mL of 0.1M MgSO4). Close the lid, and mix. What is the dilution factor? A1 - 10x (1:10), A2-...