If you transfer 1.0 ml from Sample A into 99ml of water, how would you label the water blank?
It is labelled as V/V %
Volume of sample A = 1 mL
Volume of water = 99 mL
Volume of the solution= 100 mL
V/V% = volume of sample*100/volume of the solution
= 1 * 100 / 100
= 1% (v/v)
Water blank is labelled as 1% by volume or 1% (v/v)
If you transfer 1.0 ml from Sample A into 99ml of water, how would you label...
A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to a bottle containing 99ml of sterile water. You then take 0.1 ml from this dilution bottle and transfer to a second bottle with 0.9 ml of sterile water. Finally, you transfer 1 ml to a Petri dish with nutrient agar. How many colonies would you expect to find on this plate? Would it be countable?
Show how you would make up a 10-5 dilution, given 1 ml pipets and 99ml water blanks. Your original samples is undiluted milk.
if you transfer 0.1 ml of a sample into a 99.9ml saline blank, what is the dilution?
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
You transfer 1 mL of stock solution to tube A, add 9 mL of water and mix. You then transfer 1 mL from tube A to tube B, add 9 mL of water and mix. And finally you transfer 1 mL from tube B to tube C, add 9 mL of water and mix. What is the total dilution factor for this series when comparing the stock solution with tube C? (990 is not the answer)
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a...
If 20. drops of water equall.O mL (1.0 cm3 ), (a) how many drops of water are there in a cubic mile of water? (b) what would be the volume in cubic miles of a mole of drops of water?
21. How can you make the meniscus more observable if you are having difficulty reading your buret? 22. 1.0 mL of 1.0 M HCl is added to 100 mL of a sample of pure water? 1.0 mL of 1.0M HCI is also added to 100 mL of a sample of a buffer solution. Which sample will have the greater change in pH? Explain.
How would you prepare 500 mL of a 5% D5W (dextrose in water) solution from a 29% stock solution?
Plating 1.0 ml of a sample diluted to 10^-4 produced 57 colonies. What was the CFU/ml in the original sample? What would it be if you plated 0.1 ml with same colonies formed?