Q) you will combine 15uL of each LDH purification sample with 20uL DI water and 15...
Q) you will combine 15uL of each LDH purification sample with 20uL DI water and 15 uL 4xnative gel loading buffer. what is the dilution factor of LDH sample in this mixture?
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Q) you will combine 15uL of each LDH purification sample with
20uL DI water and 15...
my questions are the following: A) what is the concentration of the stock solution of LDH?...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer...
During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 500ml of the A150 buffer?
Question 4 of 15 > If you combine 260.0 mL of water at 25.00 °C and...
Question 4 of 15 > If you combine 260.0 mL of water at 25.00 °C and 130.0 mL of water at 95.00 °C, what is the final temperature of the mixture? Use 1.00 g/mL as the density of water. Tal
3. A serial dilution of a swab sample was prepared by adding 10 uL to a...
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
a) You want to load 15 ug of protein in 10 uL into one of the...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
I just need the answers to questions 2 and 3. My DNA ladder is in lane...
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
Can you please show all calculations and any other thing you used to get the answer?...
Can you please show all calculations and any other thing you used
to get the answer? Step by step.
6. The pplied to a gel filtration column and the elution volumes determined. Generate a standard curve from this dat equation. following protein standards (Table 1) were a a. Show R2 and line Table 1. Protein mixture, Elution Volume, (mL) 20uL protein injected Thyroglobulin IgA IgG Bovine Serum Albumin Horse radish peroxidase Molecular Weight (Daltons) 670000 300000 158000 68000 40000 18.26...
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial...
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil?
1. What is the purpose of the sample buffer? 2. What would happen if you did...
1. What is the purpose of the sample buffer? 2. What would happen if you did not add the sample buffer before loading your DNA sample on the gel? 3. What is the purpose of the blue dye?
4. A 20 mL wastewater sample was mixed with 280 mL deionized (DI) water in a...
4. A 20 mL wastewater sample was mixed with 280 mL deionized (DI) water in a standard BOD bottle. The 5- day BOD test provided an initial dissolved oxygen concentration (DO intial) = 9 mg/L and a final dissolved oxygen concentration (DO final) = 3.5 mg/L. The BOD experiment was carried out at 20°C and the BOD rate constant at 20°C is 0.22/day. a. Find the dilution factor (P) and BODs value at 20°CY b. Find the ultimate BOD of...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
Question 4 of 15 > If you combine 260.0 mL of water at 25.00 °C and 130.0 mL of water at 95.00 °C, what is the final temperature of the mixture? Use 1.00 g/mL as the density of water. Tal
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
Can you please show all calculations and any other thing you used
to get the answer? Step by step.
6. The pplied to a gel filtration column and the elution volumes determined. Generate a standard curve from this dat equation. following protein standards (Table 1) were a a. Show R2 and line Table 1. Protein mixture, Elution Volume, (mL) 20uL protein injected Thyroglobulin IgA IgG Bovine Serum Albumin Horse radish peroxidase Molecular Weight (Daltons) 670000 300000 158000 68000 40000 18.26...
1. What is the purpose of the sample buffer? 2. What would happen if you did not add the sample buffer before loading your DNA sample on the gel? 3. What is the purpose of the blue dye?
4. A 20 mL wastewater sample was mixed with 280 mL deionized (DI) water in a standard BOD bottle. The 5- day BOD test provided an initial dissolved oxygen concentration (DO intial) = 9 mg/L and a final dissolved oxygen concentration (DO final) = 3.5 mg/L. The BOD experiment was carried out at 20°C and the BOD rate constant at 20°C is 0.22/day. a. Find the dilution factor (P) and BODs value at 20°CY b. Find the ultimate BOD of...
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