Can TLC or paper chromatography be used to separate and identify very volatile substances? Explain.
No, TLC or paper chromatography cannot be used to separate and identify very volatile substances. This is because developing solvent has to be evaporated off using heating process, hence volatile substance will be removed.
For separation and identification of very volatile substances HPLC or GC (high performance liquid or gas chromatography) can be used.
Can TLC or paper chromatography be used to separate and identify very volatile substances? Explain.
1.a IR spectroscopy and Thin Layer Chromatography (TLC) is very convenient in tracking reactions. Explain How you used both IR and TLC to track your reaction. Was your reaction complete when you did your initial TLC check? If so, how can you tell? 1.b Discuss how yield can be correlated to the efficacy of a reaction? Why are yields never 100%?
Question 19 Steam distillation process is used to separate substances which are Steam volatile Steam volatile and immiscible with water Steam volatile and miscible with water All of the mentioned None of the mentioned Question 20 Which of the following property is not possessed by the organic solvent required to separate an organic compound with water It is immiscible with water It is less volatile than water It is easily vaporizable Organic compound is more soluble in it None of...
Thin layer chromatography
6. When should a mixed-solvent system be used for TLC? Explain. 7. Is the order of elution of the components the same for TLC as for column chro- matography? Explain bow.the various fractions can be analyzed from a gravity column.
Liquid Chromatography can be used to separate and identify what? Metabolites DNA fragments RNA transcripts Skin cells
2. Aspirin, acetaminophen, and caffeine are some of the substances being tested in this TLC experiment. Look at their structures and atoms present to help you explain why 100% hexane would NOT be a good TLC developing solvent (chromatography solvent) to use in this Thin Layer Chromatography experiment.
How can you separate two substances with a Rf(rate of flow) value difference of less than 0.05 in the TLC?
Thin Layer Chromatography (TLC) is a common technique that is routinely used in chemistry labs. It is simple, inexpensive, and very useful. Select the TRUE statement. More polar compounds travel faster on a TLC plate than less polar compounds. If compound A has a smaller Rf value than compound B, we can conclude that compound A is more polar than B. The stationary phase of a TLC silica gel plate is nonpolar. Rr is calculated as the distance traveled...
Explain briefly how gas chromatography and mass spectrometry can be used to identify and quantitavely measure the components of an unk sample.
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC and TLC operations conducted concurrently? Explain. 3. Name a few packing materials used in packing columns in Column Chromatography operations. 4. Explain the role of eluting solvent(s) in column chromatography procedures. 5. When performing column chromatography, which of the following compounds would you expect to elute first: Ferrocene of acetylferrocene? Explain why.
A student used chromatography to separate a mixture of amino acids. Since amino acids are invisible on the paper, what can be done to make them visible?