Every cell has a remarkably complex array of processes that ensure that its genetic information is maintained and reproduced, and also ensure that the genes are manifested in the enzymes and other proteins required for the cell to function and survive. Thus, for example, an assembly of enzymes brings about the very accurate replication of DNA once during each cell cycle. Another group of enzymes and other factors catalyzes the transcription of DNA into RNA, which in turn is translated into proteins on the ribosomes. Superficially, it would seem that all these processes are far too complex to be manipulated at will in the laboratory. However, it was realized in the 1970s that it is possible to harness viruses, plasmids, and bacteria to do the manipulations on behalf of the experimental molecular biologist. A wide range of powerful and deceptively straightforward techniques has subsequently been developed. These techniques are the basis for the production of recombinant proteins
How might this transformation, or recombinant, technology be used to express rare proteins?
how DNA recombinant technology is important to produce human growth hormone with figure, explanation, and advantages
8. Explain how the LacZ gene is used in recombinant DNA technology. 9. You LOVE spinach. This morning you heard on the news that E.coli O157:H7 has contaminated 90% of the spinach crops in your area. E.coli O157://7 is a pathogenic strain of bacteria that has acquired a gene that produces a toxin that gives people hemorrhagic diarrhea. You are bummed because you CAN'T live without your spinach! You recently began working in a lab that has a PCR machine...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
how we can produce vaccine through DNA recombinant technology with explanation, figures, advantages, and examples
DNA Technology What is biotechnology? • What is genetic engineering? How does it relate to recombinant DNA? • What are genetically modified organisms (GMOs)? • What are transgenic organisms? • What are some controversies surrounding GMOs? • What do the world’s leading health agencies think about GMOs? • What are some benefits of GMOs? • How does gene therapy work? • Describe the basic steps of making recombinant DNA. • What are the two major products that you get after...
Many plasmids used to express recombinant protein also carry a gene which codes for resistance to an antibiotic. Why is such a gene often included? TTT Arial 3 (12pt) - T - = - = - S e Path: p Words:0
How current technology can be used to help people with vision problems? List some future inventions might make blindness and vision loss easier to live.
How is technology influencing teaching? How might you use technology to your advantage while teaching a patient?
How might you carry out the following transformation? (You might
need to perform a multistep synthesis)
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