how we can produce vaccine through DNA recombinant technology with explanation, figures, advantages, and examples
how we can produce vaccine through DNA recombinant technology with explanation, figures, advantages, and examples
how DNA recombinant technology is important to produce human growth hormone with figure, explanation, and advantages
DNA Technology What is biotechnology? • What is genetic engineering? How does it relate to recombinant DNA? • What are genetically modified organisms (GMOs)? • What are transgenic organisms? • What are some controversies surrounding GMOs? • What do the world’s leading health agencies think about GMOs? • What are some benefits of GMOs? • How does gene therapy work? • Describe the basic steps of making recombinant DNA. • What are the two major products that you get after...
How can you use PCR in the process of generating recombinant DNA. Why would we need to add a restriction enzyme site onto a fragment of interest in the process of generating recombinant DNA? Why would you benefit from adding two different enzyme sites to a given fragment?
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
2) With reference to production of recombinant DNA answer the following questions: i) what is "plasmid" and how is it isolated from a cell (1 point); ii) what is "insert" (1/2 point); iii) name the enzyme used for cleaving and generating sticky ends on the plasmid and insert (1 point); iv) name the enzyme used for joining sticky ends of plasmid and insert to produce a molecule of recombinant DNA (1 point); v) name the most common microbial host cell...
8. Explain how the LacZ gene is used in recombinant DNA technology. 9. You LOVE spinach. This morning you heard on the news that E.coli O157:H7 has contaminated 90% of the spinach crops in your area. E.coli O157://7 is a pathogenic strain of bacteria that has acquired a gene that produces a toxin that gives people hemorrhagic diarrhea. You are bummed because you CAN'T live without your spinach! You recently began working in a lab that has a PCR machine...
BTH2732 Recombinant DNA Technology
For answer all questions below part, Ignore question 1. Just
do question 2 and below
Part A: Refer to Lecture 3 & Supplementary Video: https://www.youtube.com/watch?v SiwNtQYLKeU Virtual cloning of the Human p53 gene You are required to devise a set of practical methodologies in order to carry out a relatively simple molecular biology task. 1. You must identify the steps required 2. Formulate a set of practical protocols needed to carry out those steps . Consider...
Discuss the IT scorecards’ advantages. Justify how the scorecard can be applied specifically to information technology. Min 175 words
8. DNA cloning. The first DNA clone using recombinant DNA technology was human insulin, which was made in 1978. The insulin gene was inserted into a plasmid and the gene was transcribed and translated from the plasmid in E coll cells. Insulin is synthesized by ribosomes in human pancreatic cells. Insulin is synthesized as a longer polypeptide, which is then trimmed by proteases to make the mature chains A and B (see Fig. 2.17 on page 39 of your textbook)....
How is PCR used in DNA cloning? Can you provide some real life examples of PCR cloning? Why/When do we use PCR over alternative cloning techniques.