Which of the following are the 3 steps involved in the basic mechanism of DNA repair?...
Homework Answers
the 3 steps involved in the basic mechanism of DNA repair are as follows
The segment of damaged strand detected and excised by DNA glycosylase and AP site- Apurine or Apyrimidine site is created which is filled by DNA polymerase.
DNA polymerase fills in the missing nucleotides by polymerization reaction using the bottom strand as a template.
After polymerization reaction the gaps are left behind which is sealed by DNA ligase.
DNA ligase seals the nick and a new repaired DNA is formed
So the correct options are a,b, c
DNA ligase does not excise the segment instead seals the segment of DNA.
Topoisomerase enzyme is not involved in polymerization reaction instead they are involved in releasing stress during replication and is not also involved in repair mechanism.
So the options d and e are wrong
Add Answer to:
Which of the following are the 3 steps involved in the basic
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And..
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Give the basic steps involved in extracting genomic
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Which are true about direct repair of DNA? O The damaged base is excised and a new segment of DNA is synthesized to fill in the gap. Error-prone DNA polymerases are used to insert a nucleotide opposite to the damaged base. O A damaged base is restored to its correct structure without removing it from the DNA. O DNA ligase is needed. O Methylation marks can be used to distinguish the correct strand from the incorrect strand.
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
21. In DNA replication, new nucleotides are added a. To the 5' end of each nascent strand b. To the 3'end of each nascent strand To both ends of each nascent strand d. To the 5' end of the continuous strand and the 3' end of the discontinuous strand e. To the end of the continuous strand and the end of discontinuous strand fragments 22. DNA unwinding is done by a. Ligase b. Helicase c. Topoisomerase dHexonuclease 23. Proofreading of...
Order steps 1-7, first step (1) and last step
(7)
Order the events in the repair of a GT base pair in E. coli. • The three-component complex (MutSLH) identifies the old strand by locating a methylation mark. The endonuclease then cleaves the unmethylated (new) strand. • The mismatch-binding protein complex (MutSL) scans the genome after DNA replication to find incorrect base pairs. • DNA polymerase extends the 3'OH of the unmethylated, new strand to synthesize new DNA. • Once...
Identify the various types of DNA repair mechanisms known to counteract the effects of UV radiation. Recombinational repair Excision repair Photoactivation repair SOS repair 1. is dependent on a photon-activated enzyme that cleaves thymine dimers. 2. is the process by which an endonuclease clips out UV- induced dimers, DNA polymerase III fills in the gap, and DNA ligase rejoins the phosphodiester backbone. 3. uses the corresponding region on the undamaged parental strand of the same polarity. 4. is a process...
And..
Exercise1:
Give the basic steps involved in extracting genomic
DNA from animal cells and tossues?
23- Which of the following statements is correct? a. Longer DNA fragments migrate farther than shorter fragments. b. Migration distance is inversely proportional to the fragment size. c. Positively charged DNA migrates more rapidly than negatively charged DNA. d. Uncut DNA migrates farther than DNA cut with restriction enzymes. 24- Why do scientists load DNA of known sizes (also called "marker" or "ladder") into...
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