Question

Explain why we expect declining in yield and % yield in beta galactosidase purification processes?

Answer 1

• β galactosidase is an enzyme which hydrolyze β-galactoside into monosaccharides through breaking the glycosidic bond.This enzyme provide energy in the body through breakdown of lactose and galactose into glucose.
• Human Lysosomal beta galactosidase(GLB1) is purified from liver cells ,human fibroblasts, and placenta.
• The mutation of GLB1 causes lysosomal storage diseases:GM₁-gangliosidosis and Morquio B disease and Galactosialidosis
• GM₁ gangliosidosis is caused by accumulation of glycolipid GM₁ ganglioside in the CNS
• Deficiency of enzyme leads to the accumulation of sphingolipid intermediates in lysosomes of neuronal tissue, resulting in the CNS deterioration typical of GM1. Deficiency of this enzyme also leads to accumulation of the glycosaminoglycan (GAG) keratan sulfate in cartilage which is suspected to cause the skeletal findings associated with MPS IVB.
• A profound deficiency of beta galactosidase is found in tissues like liver,kidney, brain and spleen.This is due to the failure to cleave both p-nitrophenyl-β-D-galactopyranoside and ganglioside GM₁ labled with C¹⁴ in the terminal galactose.It is responsible for the accumulation of ganglioside GM₁.This enzyme defect cause decline in the yield and % yield of β-galactosidase.