what is single chain variable fragment and what makes it help detect a protein like Her...
what is single chain variable fragment and what makes it help detect a protein like Her 2?
Homework Answers
Single chain variable fragment: It is a fusion protein consisting of variable regions of heavy and light chains of immunoglobulins joined together by a peptide linker( short peptide linker of 10-25 amino acids ).
They are used in detecting proteins like Her2 because:
- of their small size which facilitate the crystallographic study of cells interactions.
- they are highly immunogenic and have distinct antigens that extract antibodies specific for breast cancer.
- it can be designed as a bispecific antibody to work as immunotoxin.
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what is single chain variable fragment and what makes
it help detect a protein like Her...
Imagine a transmembrane protein consisting of a single polypeptide chain. The protein has alpha helices that...
Imagine a transmembrane protein consisting of a single polypeptide chain. The protein has alpha helices that pass through the membrane seven times. Between each segment that passes through the membrane are loops that extend into the cytoplasm or into the extracellular fluid. The C-terminus of the protein extends into the cytoplasm and the N-terminus extends into the extracellular fluid. What is the minimum number of polar stretches* in the primary structure of this protein? *polar stretches are contiguous regions of...
please help The following diagram shows a fragment of transcribed DNA, and the upper strand is...
please help
The following diagram shows a fragment of transcribed DNA, and the upper strand is the non-template strand: 5 TAACGG 3 3' ATTGCC 5 The transcribed RNA can be represented by? d. 5' UAACGG 3 c. 5' AUUGCC 3 O O b. 5 TAACGG 3 a. 5' AUUGCC 3 The primary structure of a polypeptide is: O a) the sequence of nucleotides on the DNA molecule that encodes the protein. b) the linear sequence of amino acids that constitutes...
Immunology Help 1) Expression of functional beta chain protein signals that a thymocyte has successfully reached...
Immunology Help 1) Expression of functional beta chain protein signals that a thymocyte has successfully reached what stage of development? A The pro-T cell stage B The pre-T cell stage C The double-negative stage D The double-positive stage E The single-positive stage 2) Which one of the following types of antibodies is least likely to be found in normal lymph fluid? A IgA B IgD C IgE D IgG E IgM 3) Which CDR is found closest to the center...
Based on the discussed protein purification/characterization techniques which chain of experiments makes logical sense if one...
Based on the discussed protein purification/characterization techniques which chain of experiments makes logical sense if one wants to study protein structure? a. Cell/tissue homogenization->Affinity chromatography->Mass-Spec-> Gel filtration chromatography b. Cell/tissue homogenization->Mass-Spec->Gel filtration chromatography->Affinity chromatography c. Cell/tissue homogenization->Gel filtration chromatography->Affinity chromatography->Mass-Spec d. Gel filtration chromatography->Cell/tissue homogenization->Affinity chromatography->Mass-Spec
What happens to actual carbon chain of protein if person eat more protein than body needs?
What happens to actual carbon chain of protein if person eat more protein than body needs?
What happens if the spliceosome makes a small mistake, such as inserting or deleting a single...
What happens if the spliceosome makes a small mistake, such as inserting or deleting a single nucleotide from the final spliced mRNA? - Can mutations in the DNA destroy a splice site? What is the consequence for the protein if and when this happens? - Can mutations in the DNA create new splice sites? If so, what is the consequence for the protein?
Protein A binds to the IgGs of different species of animals with variable affinity. What does...
Protein A binds to the IgGs of different species of animals with variable affinity. What does this difference tell you about the Protein A epitope? a. the epitopes of the species are identical b. Some species lack IgGs and therefore cannot bind to Protein A c. the epitopes are similar, but with enough difference that it makes the affinity variable. d. The epitopes are very different in all the species Provide reasoning with your answer. d.
what are the chemical shifts , multiplicity, neighboring H1, integration, and possiblr functionality or chain fragment?...
what are the chemical shifts , multiplicity, neighboring H1,
integration, and possiblr functionality or chain fragment?
this is for HNMR
abundance 0 1.0 2.0 3.0 4.0 5.0 6.0 2.0 .0 0.0 10.0 11.0 12.0 13.0 14.0 Alkene X: parts per Million: 1 16 15 14 finght 13 12 11 10 09 08 hydrogen 07 methyl ? Algune Alkhalide 06 05 next to 04 03 a 3.5 02 01
An exoenzyme that hydrolizes a protein is called a _____________. The emergence of a single vegetative...
An exoenzyme that hydrolizes a protein is called a _____________. The emergence of a single vegetative bacterium from an endospore. If SIM medium turns black after inoculation and incubation, what has the bacterium produced? (2 words) This structure helps many pathogenic and normal flora bacteria to initially resist phagocytosis by the host's phagocytic cells. In soil and water, capsules help prevent bacteria from being engulfed by protozoans. It also helps many bacteria to adhere to surfaces and thus resist flushing....
please i need help with a, b, c this is the sequence 5’ATGTATTATTATTTTTTTGTTTTTTTTGCAATATATGCTAATGGATTGCTAAGAAATA AAGATCCTAACATTTTTGCGAG TAGCAATGATGAGATCATAGAAAATGATAAAAGTATGAATACCTTTGTTATGTCAAC AAATGGAAGTTTATATTTAAATA...
please i need help with a, b, c
this is the sequence
5’ATGTATTATTATTTTTTTGTTTTTTTTGCAATATATGCTAATGGATTGCTAAGAAATA
AAGATCCTAACATTTTTGCGAG
TAGCAATGATGAGATCATAGAAAATGATAAAAGTATGAATACCTTTGTTATGTCAAC
AAATGGAAGTTTATATTTAAATA
GTGATTTTAATTTAAATGAAGCATCCAACGAAAGCTTCTTAGAAAATTGCAATATCA
ATAGTTGTGTAGATATAGGTCAT
GAAAATGGCAACAAAATAAATAGTCAAGAAAATGAGCATGCTAAAAATAATAACA
ACAGTAATAATAACAATTTAAAACC
AGAATACAATAATAATAATAATAATTTAAAACCAGAATACAATAATAATAATTTAA
AACCAGAGTACAATAATAACAATT-3’
1. Polymerase chain reaction 5'- ATGTATTATTATTTTTTTGTTTTTTTTGCAATATATGCTAATGGATTGCTAAGAAATA AAGATCCTAACATTTTTGCGAG TAGCAATGATGAGATCATAGAAAATGATAAAAGTATGAATACCTTTGTTATGTCAAC AAATGGAAGTTTATATTTAAATA GTGATTTTAATTTAAATGAAGCATCCAACGAAAGCTTCTTAGAAAATTGCAATATCA ATAGTTGTGTAGATATAGGTCAT GAAAATGGCAACAAAATAAATAGTCAAGAAAATGAGCATGCTAAAAATAATAACA ACAGTAATAATAACAATTTAAAACC AGAATACAATAATAATAATAATAATTTAAAACCAGAATACAATAATAATAATTTAA AACCAGAGTACAATAATAACAATT-3' a) One strand of a chromosomal DNA sequence is shown above. How would you amplify and isolate a DNA fragment defined by the sequence shown in red, using polymerase chain reaction. Design PCR primers (Forward and Reverse primers, each 20 nucleotides long, that...
please help
The following diagram shows a fragment of transcribed DNA, and the upper strand is the non-template strand: 5 TAACGG 3 3' ATTGCC 5 The transcribed RNA can be represented by? d. 5' UAACGG 3 c. 5' AUUGCC 3 O O b. 5 TAACGG 3 a. 5' AUUGCC 3 The primary structure of a polypeptide is: O a) the sequence of nucleotides on the DNA molecule that encodes the protein. b) the linear sequence of amino acids that constitutes...
what are the chemical shifts , multiplicity, neighboring H1,
integration, and possiblr functionality or chain fragment?
this is for HNMR
abundance 0 1.0 2.0 3.0 4.0 5.0 6.0 2.0 .0 0.0 10.0 11.0 12.0 13.0 14.0 Alkene X: parts per Million: 1 16 15 14 finght 13 12 11 10 09 08 hydrogen 07 methyl ? Algune Alkhalide 06 05 next to 04 03 a 3.5 02 01
please i need help with a, b, c
this is the sequence
5’ATGTATTATTATTTTTTTGTTTTTTTTGCAATATATGCTAATGGATTGCTAAGAAATA
AAGATCCTAACATTTTTGCGAG
TAGCAATGATGAGATCATAGAAAATGATAAAAGTATGAATACCTTTGTTATGTCAAC
AAATGGAAGTTTATATTTAAATA
GTGATTTTAATTTAAATGAAGCATCCAACGAAAGCTTCTTAGAAAATTGCAATATCA
ATAGTTGTGTAGATATAGGTCAT
GAAAATGGCAACAAAATAAATAGTCAAGAAAATGAGCATGCTAAAAATAATAACA
ACAGTAATAATAACAATTTAAAACC
AGAATACAATAATAATAATAATAATTTAAAACCAGAATACAATAATAATAATTTAA
AACCAGAGTACAATAATAACAATT-3’
1. Polymerase chain reaction 5'- ATGTATTATTATTTTTTTGTTTTTTTTGCAATATATGCTAATGGATTGCTAAGAAATA AAGATCCTAACATTTTTGCGAG TAGCAATGATGAGATCATAGAAAATGATAAAAGTATGAATACCTTTGTTATGTCAAC AAATGGAAGTTTATATTTAAATA GTGATTTTAATTTAAATGAAGCATCCAACGAAAGCTTCTTAGAAAATTGCAATATCA ATAGTTGTGTAGATATAGGTCAT GAAAATGGCAACAAAATAAATAGTCAAGAAAATGAGCATGCTAAAAATAATAACA ACAGTAATAATAACAATTTAAAACC AGAATACAATAATAATAATAATAATTTAAAACCAGAATACAATAATAATAATTTAA AACCAGAGTACAATAATAACAATT-3' a) One strand of a chromosomal DNA sequence is shown above. How would you amplify and isolate a DNA fragment defined by the sequence shown in red, using polymerase chain reaction. Design PCR primers (Forward and Reverse primers, each 20 nucleotides long, that...
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