Question

1. List and discuss the three microscope parts that affect the light that passes through your specimen. Explain the function of each microscope part involved, and the adjustments you might make to each while viewing a slide.

2. Write a list of steps to outline the procedure you must follow to view specimens a high power magnification. Start with a slide in place on a lowered stage, but omit light adjustments.

3. Explain why you should center the specimen before switching to higher magnification.

4. Why might you need to construct a 3-D image of a specimen at high power? Describe how you would do this in practice.

5. List everything you should do in order to put your microscope away properly.

Answer 1

1) A light source is important to see objects under microscope. These source of light should be focused properly to view sample clearly.

Illuminator act as the light source by which a light is coming from the base of the microscope get on to the sample.

- Include LED ring lights, halogen fiber optic lights and dual pipe lights.

- Provides a steady source of light

- It is placed in place of mirror if mirror is not present there

Iris diaphragm or Iris is a circular opening that controls the amount of to be incident on the slide.

- located above the condenser and below the stage

- It is rotating disk to adjust the amount of light.

- contain different sized holes that varies the intensity of light.

- Closing of diaphragm increases the amount of contrast

Mirror is used in microscope reflect light off an external source onto the slide. Moving the mirror will change the illumination of light on to the slide.