Why is PCR required before running the DNA on a gel? A Without PCR, there would...
Why is PCR required before running the DNA on a gel?
| A |
Without PCR, there would be things in the DNA that would interfere with running it on the gel. |
|
| B |
Without PCR, there would be too little of the DNA region of interest to see it on the gel. |
|
| C |
Without PCR, the DNA region of interest would not be present because PCR adds the part that will be examined. |
|
| D |
Without PCR, the gel would not have a matrix that would separate the DNA. |
Homework Answers
Option B
note- pcr is used for polymerising the dna strand into large number . This process allow the larger volume of dna to separated on the gel of electrophoresis thus pcr is done before it as it is very small in quantity to be separated before pcr.
Add Answer to:
Why is PCR required before running the DNA on a gel?
A
Without PCR, there would...
1. Explain why, when PCR is used to amplify the same region of DNA from two...
1. Explain why, when PCR is used to amplify the same region of DNA from two different people, the size of the DNA fragment(s) generated may be different? 2. What characteristic of the DNA molecule makes it possible to use electrophoresis to separate DNA molecules by size? Explain why this characteristic is important for electrophoresis and what part of the DNA molecule creates this characteristic. 3. You are performing PCR. After four cycles of PCR, how many double-stranded copies of...
estrogen + estrogen estrogen + estrogen estrogen + estrogen (fig68) anti-ER anti-H3K27AC Input DNA PCR: estrogen...
estrogen + estrogen estrogen + estrogen estrogen + estrogen (fig68) anti-ER anti-H3K27AC Input DNA PCR: estrogen response element (ERE) in black region Set 1 Set 2 Set 3 Because MYC showed higher expression in the ovarian tumor exposed to estrogen, you suspect MYC may be regulated by the estrogen hormone receptor, a nuclear receptor. To see if the estrogen receptor binds to the enhancer region in the tumor cells, you perform ChIP-PCR. The figure above shows three different sets of...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-5’ and template above. b. The primers in part b were designed to make it easy to illustrate the PCR process, in practice PCR primers are 18-25bp in length. Why do PCR primers that are 5bp fail? c. Are primers used in PCR RNA or DNA? d. Explain how PCR amplifies the gene of interest without amplifying the...
Using the PCR Thermocycler, the DNA sample was heated to what temperature? Group of answer choices...
Using the PCR Thermocycler, the DNA sample was heated to what temperature? Group of answer choices 99 Degrees Celsius 90 Degrees Celsius 95 Degrees Celsius 88 Degrees Celsius Flag this Question Question 21 pts We also used the microcentrifuge to spin our sample of cheek cells. Why is it important to balance the microcentrifuge? Group of answer choices Running a centrifuge with unbalanced load could permanently damage the centrifuge. It could also cause injury to you or someone else. Perfect...
Can someone explain this, I do not understand, I have the answer but I just don't understand why? Question 1. Consider the segment of DNA below, taken from several CCNY rattus. From each rat,...
Can someone explain this, I do not understand, I have
the answer but I just don't understand why?
Question 1. Consider the segment of DNA below, taken from several CCNY rattus. From each rat, the fragment was amplified using the primers shown. Some of the amplification product was immediately run on a DNA agarose gel: the remainder was treated with EcoRI, then run on a separate gel. On the gel representation below, show where each DNA fragment would migrate for...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A....
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
estrogen + estrogen estrogen + estrogen estrogen + estrogen (fig68) anti-ER anti-H3K27AC Input DNA PCR: estrogen response element (ERE) in black region Set 1 Set 2 Set 3 Because MYC showed higher expression in the ovarian tumor exposed to estrogen, you suspect MYC may be regulated by the estrogen hormone receptor, a nuclear receptor. To see if the estrogen receptor binds to the enhancer region in the tumor cells, you perform ChIP-PCR. The figure above shows three different sets of...
Can someone explain this, I do not understand, I have
the answer but I just don't understand why?
Question 1. Consider the segment of DNA below, taken from several CCNY rattus. From each rat, the fragment was amplified using the primers shown. Some of the amplification product was immediately run on a DNA agarose gel: the remainder was treated with EcoRI, then run on a separate gel. On the gel representation below, show where each DNA fragment would migrate for...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
13. Why are ribonucleoside triphosphates the monomers required for RNA synthesis rather than ribonucleoside monophosphates? A. Only ribonucleoside triphosphates contain the sugar ribose. B. Ribonucleoside triphosphates have low potential energy, making the polymerization reaction endergonic. C. Ribonucleoside triphosphates have high potential energy, making the polymerization reaction exergonic. D. Ribonucleoside monophosphates cannot form complementary base pairs with the DNA template. E. Ribonucleoside triphosphates are not used, rather all use deoxyriboside triphosphates. 14. How is a mutation in a bacterial cell that...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Most questions answered within 3 hours.
-
According to Lucas and Sargent, workers and firms have
rational expectations, and therefore if the Fed...
asked 1 second from now -
In the United States, each bank panic in the late nineteenth and
early twentieth centuries was...
asked 13 minutes ago -
q1. Variable costs change in direct proportion to a change in
the activity level. true or...
asked 21 minutes ago -
The velocity of a particle moving along the x axis is given for
t > 0...
asked 43 minutes ago -
A 2.5 gram penny is given a charge of -4.0 *10^-9 c. How many
excess electrons...
asked 34 minutes ago -
If businesses are pessimistic, they are _____.
moving up, along the investment demand curve
more likely...
asked 41 minutes ago -
Read the information provided. Then explain which law(s)
(could be more than one law) would protect...
asked 42 minutes ago -
Write a function named getComputerinfo that displays disk usage,
memory usage, the uptime, currently logged users,...
asked 58 minutes ago -
If I could have some assistance with the following questions
that would be appreciated. If I...
asked 59 minutes ago -
Under IFRS, which of the following subtotals would be shown on
an income statement if the...
asked 53 minutes ago -
_________________ is one of the algorithms that TCP uses to
control congestion inside the network. It...
asked 1 hour ago -
Consider an arbitrary curve of fixed length C that connects the
origin and a point a...
asked 1 hour ago