1. During the extraction process, the protein samples must be kept on ice. Why? 2. What...
1. During the extraction process, the protein samples must be kept on ice. Why?
2. What is the role of the bovine serum albumin (BSA) in this experiment?
3. Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL.
Homework Answers
1. During extraction cells are lysed with the homogenizer that produce heating effect in the solution that may denature the proteins , so to prevent heat generation and proteins from Denaturation , proteins samples are kepti in ice.
2. BSA is used to plot a standard curve of known concentration of proteins. From this standard curve we can calculate the concentration of unknown protein.
3. For this, please provide the concentration of BSA stock and number and concentration of dillutions .
Add Answer to:
1. During the extraction process, the protein samples must be
kept on ice. Why?
2. What...
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please help with my pre lab additional information Pre-Lab Questions: 1. What is the definition of...
please help with my pre lab
additional information
Pre-Lab Questions: 1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCI stock solution. In your response to this question, be very specific about the quantities of stock solution and...
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Please show work:
1) Complete the calculations and fill in the missing volumes needed below to create a six-tube solute dilution series from a protein stock solution, 1.0 mg/ml BSA (bovine serum albumin) into 1.5 ml microcentrifuge tubes using CIVI = C2V2, note V2 is not 1.5ml. A table is provided below to record your efforts. New solutions Tube Concentration Volumes stock protein Dl water Final volume 1.00 mg/ml 250.0 ul 0.0 ul 250.0 ul 250.0 ul 0.80 mg/ml 0.60...
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You are dissatisfied with the purity of the final preparation of
your HRP and decided to include ion-exchange (IEC) and
gel-exclusion chromatography (GEC) steps in your purification
protocol. From the literature, you found out the following info
about HRP enzyme:
Molecular weight: 40,000
Isoelectric point: 7.2
HRP is a very stable protein.
2: Design GEC purification experiment using the information in
the appendix. Address and justify the following
I. choice of GEC column
II. choice of the buffer (if you...
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