Question

Please show how to calculate Km and Vmax for no inhibitor/low inhibitor given graph. Show how to solve for a.,a/a etc.

Lineweaver Burk #4: No inhibitor, Low inhibitor 0.2 ▲ No inhibitor Low inhibitor 0.15 0.05 0.2 0.15 0.1 0.05 0.05 0.1 0.15 02 5 1/IS] in units of 1/mM Fill in the blanks. Show your work. No inhibitor Kmno Vmax,o- Vmax,w = ๙ Vmax,o Solve for ๙ inhibitor Krmkw =픕Km,o Solve for 픕 Hint treat, as a single number. Now, what is a ? Hint: plug in the number that you found for a' Write the Michaelis-Menten equation for the Low inhibitor case, using α; Kmno n Write the Michaelis-Menten equation for the Low Inhibitor case, using . Kmno inste ad of Km, and using or a' Kmno use numbers and cancel out anything that cancels What kind of inhibition is this?

Answer 1

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