Question

Based on the bacteria, please fill out the table with the true results under each circumstance. Also, tell why each ingredient is added to the bacteria and what is the purpose of it? what does it do to bacteria?

unknown was anterobacter and pseudomonas was false negative and became positive after the nitrate reduction. please answer all aspects of the quetstions to get positive feedback.

0 ys Bacteria Simmons Citrate Nitrate Broth Nutrient Slant TSA Plate pseudomonas aerugineśa Staphylococcus aureuS Enterobacter aerogene Escherichia coli Unknown

Answer 1

Bacteria	Glucose (O/F) with oil	Glucose (O/F) no oil	Glucose broth	Lactose broth	Mannitol broth	MR-VP		Simmons citrate	Nitrate broth	Nutrient slant	TSA plate
P. aeroginosa	Oxidative	No growth	Positive	Negative	Positive	Negative	Negative	Positive	Positive	Positive,blue green colonis	Positive-Circular,raised,blue-green colonies
S. aureus	Fermentative	Growth observed	Positive	Positive	Positive	Positive	Positive	Positive	Positive	Positive, yellow pigment	Positive - Yellow colonies
E. aerogenes	Fermentative	Growth observed	Positive	Negative	Positive	Negative	Positive	Positive	Positive	Positive, circular colonies	Positive, circular colonies
E.coli	Fermentative	Growth observed	Positive	Positive	Positive	Positive	Negative	Negative	Positive	Positive,large,thick greyish colonies	Positive, large colonies
Unknown	Fermentative	Growth observed	Positive	Negative	Positive	Negative	Positive	Positive	Positive	Positive, circular colonies	Positive, circular colonies

Glucose broth ( O/F) with inverted Durham's tubes: It is used to test organism's potential to ferment the sugar glucose as well as its ability to convert the end product of glycolysis, pyruvic acid into gaseous by products ( Fermntative or oxidative).

Oil/ No oil is used to check the growth under anaerobic condition. Layered mineral oil on the glucose broth prevents oxygen from diffusing to the medium.

Glucose, lactose, mannitol broth is used to test the ability of organism to utilize different sugars.

MR/VP test - This test is used to determine which fermentation pathway is used by organism to utilize glucose.

Nitrate broth - It is a differential medium which used to determine the ability of organism to reduce nitrate (NO₃^-) to nitrite (NO₂^-) or other nitrogenous compounds via the action of the enzyme nitrate reductase.  

Simmon's citrate agar - It is a defined medium used to determine the ability of organism to utilize citrate as a sole carbon source.

Tryptic Soy Agar : Tryptic Soy Agar is recommended for use as a general growth medium for the isolation and cultivation of microorganisms.