Question

Question 5 (6 pts) An enzyme exists at pH 7 as an equilibrium between hexamer and trimers. Consider the following: the specific activity of the hexamer is 30 units/mg protein; and 1 units/mg protein for the trimer // the conversion of hexamers to trimers releases 6 protons at pH 7.0 // the trimer binds sucrose, but the hexamer does not // an allosteric molecule, A,binds only to the trimer//Now answer the following question by circling one answer: a) Changing the pH from 7 to 8 would be expected to do what to the concentration of trimers increase decrease stay the same insufficient information given b) Addition of the allosteric molecule, A, will have this effect on enzyme activity increase c) addition of the allosteric molecule, A, will tend to do this to the pH of the enzyme in water increase decrease stay the same insufficient information given decrease stay the same insufficient information given d) lowering the pH from 7 to 6 would be expected to do what to the binding of the allosteric molecule A? increase decrease stay the same insufficient information given e) Changing the amount of enzyme used in the assay cuvette from 10 ug/mL to 2 ug/mL would be expected to change the specific activity of the enzyme in what way. It would. increase decrease stay the same insufficient information given f) Decreasing the concentration of A will increase decrease stay the same insufficient information given

I have the answers but I was wondering if somebody could explain them to me. thanks so much in advance!

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