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Microbiology question: Predict the structure arising from homologous recombination using insertio...

Microbiology question: Predict the structure arising from homologous recombination using insertion sequences located on both a circular plasmid and the bacterial chromosome.

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MUTANT 1 MUTANT2 TSB Tlobo 133A model to explain the heteroduplex DNA structure shown in Figure 2. IS1 is represented by the dark lines; α and β represent the ends of the IS1 molecule; H and L represent the strands of λd gal DNA with high and low buoyant densities respectively. The hybrid can be explained by assuming that the IS1 sequence is inserted in opposite directions in the two mutants.

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