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6). TrisHCl is an organic buffering compound that forms an equilibrium with its conjugate base (TrisCl-) according to th...

6). TrisHCl is an organic buffering compound that forms an equilibrium with its conjugate base (TrisCl-) according to the following equilibrium.
TrisHCl TrisCl- + H+ pKa= 8.1 -

  1. (i) In a solution which has a pH=8 what are molar ratios of TrisCl of TrisHCl l?

  2. (ii) If the above solution which has a total Tris concentration ([TrisHCl] + [TrisCl-] =

    1 mol L-1) of 1 mol L-1 what are the concentrations of TrisHCl and TrisCl?

  3. (iii) What masses of TrisHCl and TrisCl are required to make a 1L solution with these

    calculated concentrations?

Molecular weight of TrisHCl= 157.6 g mol-1

Molecular weight of TrisCl- = 121.14 g mol-1

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