How many turns are in a B-form DNA fragment that is 714 nm long?
In a b form dna the length of dna per turn is 3.4 angstrom or 34 nm
Given length of dna is 714nm
Hence number of turns =714/34 = 21 turns
If DNA has 3,400,000 base pairs, how many turns does it have?
You are interested in knowing something about a fragment of DNA that is 10 Kb long. You decide to build a restriction map of the DNA fragment, and cut up the DNA using some restriction enzymes shown below. After running the restriction digests (cut DNA) out on a gel you get the following results: 6. 10 8.5 6.5 6.0 e 4.5 4.0 3.5 2.0 1.5 Stained Gel Develop a restriction map placing the restriction enzymes used in the most logical...
A 52.0 nm-long duplex DNA molecule in the B-conformation adopts the A-conformation upon dehydration. What is its approximate number of base pairs? base pairs How long is it after dehydration? nm
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
A short fragment of a DNA molecule is 9,638,000 nucleotides in length and includes 50,994 nucleosomes. How long is the linker DNA between the nucleosomes?
Suppose you start a PCR reaction with 3 copies of a double stranded DNA fragment. How many copies will be present after 4 replication cycles? a. 7 b. 64 c. 48 d. 24 e. 8
If you cut the following single stranded DNA fragment with a restriction enzyme with restriction site of 5’GAATTC 3” and the cutting point between G and A. a. How many fragments you will get b. Specify the size (Number of bases) and the sequence of each fragment, pay attention to DNA direction (5’-3’) 5” ACATTGTCCGGGAATTC CGGGCTAGGCAT T GAATTGGAACA GAATTC GGGCCCGATCCGTA 3
If a 1000 kilo base fragment of DNA has five evenly spaced replication origins and DNA polymerase moves at 1 kilo base per second, how many seconds will a cell take to produce two daughter molecules
You have isolated a DNA fragment containing a human gene involved in lung cancer and are working inserting it into a standard plasmid vector used in molecular cloning which has a multiple cloning site in the lacZ gene. After cutting the DNA fragment and the vector with the same restriction enzyme and ligating the cut DNA, you transform bacteria with hopefully the recombinant vector. How do you expect to identify bacteria with the recombinant vector? A. Bacteria with the recombinant...
6. Answer the following using numerical values: a. A double-stranded DNA molecule is 300,000 basepairs long. This DNA molecule has how many complete helical turns? (Use a numerical answer, no words or units.) b. A double-stranded DNA molecule is 300,000 basepairs long. How long is the DNA molecule in micrometers? (Use a numerical answer, no words or units.)