A 52.0 nm-long duplex DNA molecule in the B-conformation adopts the A-conformation upon dehydration. What is...
If 50.0 % of the base pairs in a duplex DNA molecule (12.5 kbp) are in the B-conformation and 50.0 % are in the Z-conformation, what is the long dimension of the molecule?
[References If 34.0% of the base pairs in a duplex DNA molecule (12.5 kbp) are in the B-conformation and 66.0 % are in the Z-conformation, what is the long dimension of the molecule?
Shown below are the sequences of three duplex DNA molecules. I. 5" AGCCTAACTGCGAT 3' II. 5' CGCATTATTGTCAA 3' III. 5' CTAACCCGCACTGG 3' 3' TCGGATTGACGCTA 5' 3' GCGTAATAACAGTT 5' 3' GATTGGGCGTGACC 5' a. State the relationship between the percentage of AT base pairs in a double-stranded DNA molecule and the melting point of that molecule. b. Order the above three molecules (I, II, III) in terms of their melting points from lowest to highest. The graph below shows the results of...
Chromosome 3 contains nearly 200 million nucleotide pairs of our genome. If this DNA molecule could be laid end to end, how long would it be? The distance between neighboring base pairs in DNA is typically around 0.34 nm. About 7 mm About 7 cm About 7 m None of the above
A double-stranded DNA molecule of 175 base pairs long had (A+T/(G+C) = 0.75. How many adenine nucleotides are there in this DNA? 75 37.5 150
You are given a circular DNA molecule to analyze that is 3,000bp (3 Kb) long. You proceed to treat the DNA molecule with different cutting enzymes and then you run the individual reactions on a gel. The following gel is produced with each column representing a different reaction (lane). In lane 1 a molecule weight ladder is run. In lane 2, the circular DNA is NOT treated with any enzyme. In lane 3 the DNA is treated with an enzyme...
A relaxed B-type double stranded circular DNA molecule of 8,400 base pairs was cleaved by an E. coli Type II topoisomerase. What was the original linking number (Lko) and the final linking number (Lk) for this DNA molecule? a. Lko=1,200; Lk=1,199 b. Lko=800; Lk=802 c. Lko-400; Lk=401 d. Lko+800; Lk=798 e. Lko=400; Lk=399 An explanation of the answer would be appreciated, thanks! The rate of bp/turn = 10.5.
How many turns are in a B-form DNA fragment that is 714 nm long?
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively charged (with a charge of two electrons per base pair). When you “run the gel” you are generating an electric field by connecting anodes and cathodes at the ends of the gel. This causes the negatively charged DNA segments to move towards the positive electrode. After running the gel, smaller DNA segments have moved farther from the...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...