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You are interested in knowing something about a fragment of DNA that is 10 Kb long....
A linear fragment of DNA is cleaved with the individual restriction enzymes Hindill and Smal, and...
A linear fragment of DNA is cleaved with the individual restriction enzymes Hindill and Smal, and then with a combination of the two enzymes. The fragments obtained are: Eco RV 2 kb, 3.5 kb, 9.5 kb Bam HI 6 kb, 9 kb Eco RV and Bam H12 kb, 3.5 kb, 4 kb. 5.5 kb Draw a restriction map of the DNA fragment. (5 marks). Model of example restriction map to show you how to give your answer: Eco R1 Hind...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb 4 kb -> 1 kb 2 kb 3 kb You decided to set up an experiment where you added one of these restriction enzymes to this DNA. After this DNA was digested with that restriction enzyme, you separated the resulting fragment(s) using agarose electrophoresis. After the gel was stained with ethidium bromide, you observed the following gel (the DNA ladder is your reference standard...
A linear piece of DNA has the following restrictions sites: You decided to set up an...
A linear piece of DNA has the following restrictions sites:
You decided to set up an experiment where you added one of these
restriction enzymes to this DNA. After this DNA was digested with
that restriction enzyme, you separated the resulting fragment(s)
using agarose electrophoresis. After the gel was stained with
ethidium bromide, you observed the following gel (the DNA ladder is
your reference standard and is comprised of a series of DNA
fragments of known length).
a) Which restriction...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes Pvu...
Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes PvuII, HincII, ClaI, and BanII, alone and in pairs. The digestion products are separated by gel electrophoresis, and the size of each fragment is determined by comparison to size standards. The fragment sizes obtained, in kilobase pairs, are given below. Draw a diagram of the 7.8-kb fragment, showing the location of the restriction sites for each enzyme. a.Digestion with PvuII: 1.3 kb, 6.5 kb b.Digestion...
You have determined that a bacterial strain you are working with contains a single type of...
You have determined that a bacterial strain you are working with
contains a single type of plasmid. You isolate the plasmid DNA and
digest separate portions of it with each of two different
restriction enzymes, BamHI and HpaI, and also perform a double
digest using both enzymes. You then fractionate the enzyme digests
on an agarose gel and stain the gel with ethidium bromideto
visualize the restriction fragment patterns. Your results are shown
below. Size markers (in nucleotide base pairs)...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160 kb long: Enzyme A cuts at location(s): 20 kb, 45 kb 70 kb and 110 kb. Enzyme B cuts at location(s): 15 kb and 140 kb. Based on this information, first construct a linear map of this DNA showing the positions of these restriction enzyme cut sites. In the space below.draw what the gel would look like given the following lanes: (8 points total)...
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following...
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following data Hind iii..... 3kb, 7kb BamHi ... 2 kb and 8kb HInd iii + bamHI ..... 1kb, 2kb, 7kb Draw a restriction map of this DNA fragment, labeling the sites for EcoRI, bahHI, and HIndiii
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
5. You have a linear DNA fragment and you wish to generate a restriction map using...
5. You have a linear DNA fragment and you wish to generate a restriction map using Mstl and EcoR1. When the framgent is digested with Mst1 and the DNA is run on a gel you observe a 4kb and a 6kb fragment. When the DNA is digested with EcoR1, 1kb, 4kb and 5kb fragments are generated, A double digest using both enzymes produce 1kb, 2kb, 3kb and 4kb fragments. Explain these results and draw a restirction map consistent with these...
A linear fragment of DNA is cleaved with the individual restriction enzymes Hindill and Smal, and then with a combination of the two enzymes. The fragments obtained are: Eco RV 2 kb, 3.5 kb, 9.5 kb Bam HI 6 kb, 9 kb Eco RV and Bam H12 kb, 3.5 kb, 4 kb. 5.5 kb Draw a restriction map of the DNA fragment. (5 marks). Model of example restriction map to show you how to give your answer: Eco R1 Hind...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb 4 kb -> 1 kb 2 kb 3 kb You decided to set up an experiment where you added one of these restriction enzymes to this DNA. After this DNA was digested with that restriction enzyme, you separated the resulting fragment(s) using agarose electrophoresis. After the gel was stained with ethidium bromide, you observed the following gel (the DNA ladder is your reference standard...
A linear piece of DNA has the following restrictions sites:
You decided to set up an experiment where you added one of these
restriction enzymes to this DNA. After this DNA was digested with
that restriction enzyme, you separated the resulting fragment(s)
using agarose electrophoresis. After the gel was stained with
ethidium bromide, you observed the following gel (the DNA ladder is
your reference standard and is comprised of a series of DNA
fragments of known length).
a) Which restriction...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
You have determined that a bacterial strain you are working with
contains a single type of plasmid. You isolate the plasmid DNA and
digest separate portions of it with each of two different
restriction enzymes, BamHI and HpaI, and also perform a double
digest using both enzymes. You then fractionate the enzyme digests
on an agarose gel and stain the gel with ethidium bromideto
visualize the restriction fragment patterns. Your results are shown
below. Size markers (in nucleotide base pairs)...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160 kb long: Enzyme A cuts at location(s): 20 kb, 45 kb 70 kb and 110 kb. Enzyme B cuts at location(s): 15 kb and 140 kb. Based on this information, first construct a linear map of this DNA showing the positions of these restriction enzyme cut sites. In the space below.draw what the gel would look like given the following lanes: (8 points total)...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
5. You have a linear DNA fragment and you wish to generate a restriction map using Mstl and EcoR1. When the framgent is digested with Mst1 and the DNA is run on a gel you observe a 4kb and a 6kb fragment. When the DNA is digested with EcoR1, 1kb, 4kb and 5kb fragments are generated, A double digest using both enzymes produce 1kb, 2kb, 3kb and 4kb fragments. Explain these results and draw a restirction map consistent with these...
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