Design the experiment to prove oxyR function(descript delete, over express, EMSA, RNA signal etc.)
Design the experiment to prove oxyR function(descript delete, over express, EMSA, RNA signal etc.)
Homework Answers
oxyR encodes hydrogen peroxide - inducible gene activator.
This protein helps in regulation of hydrogen peroxide inducible genes such as ahpC, F, oxyS, fur and grx etc. OxyR also helps in formation of surface proteins. In this way, these ptoteins helps the aggregation ability of E. coli cells. This leads to give a morphology to the colony of E. coli.
If the gene is deleted, then hydrogen peroxide gene are not regulated. If hydrogen peroxide is not regulated, then E. coli immunity decreases. And colony aggregation do not take place.
If the gene is overexpressed, then in single colony, there are found many number of E.coli.
OxyR us regulated hy grx protein. This protein leads to reduction of disulfide bond in oxyR. In this way, grx regulated oxyR via negative regulation whereas oxyS activate the expression of oxyR.
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Design the experiment to prove oxyR function(descript delete, over express, EMSA, RNA signal etc.)
can anyone solve this question for me please! -5 2. Express the signal x(t) as an...
can anyone solve this question for me please!
-5 2. Express the signal x(t) as an exponential Fourier series. The time variable is in units of seconds. Use MATLAB to generate the signal x(t) as a partial sum of sinusoidal terms. Plot the resulting signal over a time interval that is at least three periods long. Generate a spectral plot showing separately magnitude as a function of k and phase as a function of k
Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would...
Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?
Design an 8-function ALU that accepts 4-bit inputs a and b, a 3-bit input signal select,...
Design an 8-function ALU that accepts 4-bit inputs a and b, a 3-bit input signal select, and produces a 5-bit output out. The ALU implements the following functions based on 3-bit input signal select. - - - - - - - select signal function 3’b000 out = a 3’b001 out = a+b 3’b010 out = a-b 3’b011 out = a/b 3’b100 out = a%b (remainder) 3’b101 out = a << 1 3’b110 out = (a>b) (magnitude comparison) 3’b111 out =...
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Logic design
Experiment 3 Design with Decoders and Multiplexers 1. Function Set Assignment Function set number F(wxya)-E m(e, 5,6,9, 13,15)+d,z,s,lo) Fs(wx.ya)Cy +u'+2)(x +y 2 2. Design Procedures Fxw.xya)-Em, 5,10,12,13,14, 1s (Show the implementation of F, and F by a 74155 IC and some external gates. Draw a circuit diagram.) 155 15o C Y3 b12 Y2 YO Y2 13 Y1 YO Draw the sub-function K-maps for F3 with w, x, z as expansion variables. Based on the sub-function K-maps, the data...
B1) Prove that the function f(x,y) = c=y 0 otherwise, is integrable over [0, 1] x...
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Consider the signal f[k] illustrated below 3 2 1 (a) Using the definition, provide the DTFT of flk]. (b) Express f [kas a rect function. Use the DTFT pair to solve for the DTFT (it should look a lot...
Consider the signal f[k] illustrated below 3 2 1 (a) Using the definition, provide the DTFT of flk]. (b) Express f [kas a rect function. Use the DTFT pair to solve for the DTFT (it should look a lot different from the previous solution) c) Verify that both of your solutions are, in actuality, the same by evaluating them at 0, T/2, T
Consider the signal f[k] illustrated below 3 2 1 (a) Using the definition, provide the DTFT of...
Design a CMOS gate implementing the function F = ((?*?)+?) (with a NOT over the whole...
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Describe how you would design the constructs for this experiment in which you repair the mutations in WAS, conferring normal immune system function and blood clotting.
In humans, Wiskott-Aldrich syndrome is characterized by abnormal immune system function (immune deficiency) and a reduced ability to form blood clots. Mutations in a single gene, WAS, gene confer Wiskott-Aldrich syndrome. You study it and use CRISPR-Cas9. Describe how you would design the constructs for this experiment in which you repair the mutations in WAS, conferring normal immune system function and blood clotting. Include all relevant constructs you’d need to make, and what model organism you would use
Q8*. (15 marks) The following f(t) is a periodic function of period 2π defined over the domain when 0 < t < t π f (t) When π Express f(t) as a Fourier series expansion Q8*. (15 marks) The...
Q8*. (15 marks) The following f(t) is a periodic function of period 2π defined over the domain when 0 < t < t π f (t) When π Express f(t) as a Fourier series expansion
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can anyone solve this question for me please!
-5 2. Express the signal x(t) as an exponential Fourier series. The time variable is in units of seconds. Use MATLAB to generate the signal x(t) as a partial sum of sinusoidal terms. Plot the resulting signal over a time interval that is at least three periods long. Generate a spectral plot showing separately magnitude as a function of k and phase as a function of k
Design an 8-function ALU that accepts 4-bit inputs a and b, a 3-bit input signal select, and produces a 5-bit output out. The ALU implements the following functions based on 3-bit input signal select. - - - - - - - select signal function 3’b000 out = a 3’b001 out = a+b 3’b010 out = a-b 3’b011 out = a/b 3’b100 out = a%b (remainder) 3’b101 out = a << 1 3’b110 out = (a>b) (magnitude comparison) 3’b111 out =...
Logic design
Experiment 3 Design with Decoders and Multiplexers 1. Function Set Assignment Function set number F(wxya)-E m(e, 5,6,9, 13,15)+d,z,s,lo) Fs(wx.ya)Cy +u'+2)(x +y 2 2. Design Procedures Fxw.xya)-Em, 5,10,12,13,14, 1s (Show the implementation of F, and F by a 74155 IC and some external gates. Draw a circuit diagram.) 155 15o C Y3 b12 Y2 YO Y2 13 Y1 YO Draw the sub-function K-maps for F3 with w, x, z as expansion variables. Based on the sub-function K-maps, the data...
B1) Prove that the function f(x,y) = c=y 0 otherwise, is integrable over [0, 1] x [0, 1].
Consider the signal f[k] illustrated below 3 2 1 (a) Using the definition, provide the DTFT of flk]. (b) Express f [kas a rect function. Use the DTFT pair to solve for the DTFT (it should look a lot different from the previous solution) c) Verify that both of your solutions are, in actuality, the same by evaluating them at 0, T/2, T
Consider the signal f[k] illustrated below 3 2 1 (a) Using the definition, provide the DTFT of...
Q8*. (15 marks) The following f(t) is a periodic function of period 2π defined over the domain when 0 < t < t π f (t) When π Express f(t) as a Fourier series expansion
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