Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would...
Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?
Homework Answers
Try potato catalase, which runs the reaction of the
reduction of hydrogen peroxide to oxygen and water. The substrate
for catalase is hydrogen peroxide.
Just cut up a potato, and immerse it in hydrogen peroxide, and it
begins to fizz as oxygen is produced.
or
The best enzymes you should use is kinoallase, which is both easy to obtain and use. It's been widely studied by scientists, and has an optimal temperature range from 12 degrees celsius to 81 degrees, a 69 degree range. There are actually two different substrates that work best with kinoallase. They are formaldehyde and nitrogen triiodide . For controls, you could use the deactivated form of it, known as phenoallase, which is activated when it reacts with human semen. To buy some, ask for it at Walgreens or CVS. It's also known as Rohypnol, or "roofies" in street parlance.
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Design an experiment to determine the optimal temperature for
enzyme function, complete with controls. Where would...
Enzymes Temperature is an important factor that can regulate enzyme-mediated functions. Temperature is one of the...
Enzymes Temperature is an important factor that can regulate enzyme-mediated functions. Temperature is one of the tightly homeostatically controlled variables in the human body. What are the symptoms of loss of temperature control during a high fever? Explain why these symptoms occur based on enzyme function and cell signaling effects that are dependent on temperature. Based on Figure 1, explain how the temperature optimum of the activity of enzymes is expected to differ in organisms depending on where they live....
POSTLAB QUESTIONS Name: Masa Ahmad 1. What were the optimal pH, temperature and enzyme concentration for...
POSTLAB QUESTIONS Name: Masa Ahmad 1. What were the optimal pH, temperature and enzyme concentration for catalase? 2. Define the term denatured. Denatured is the Breaking the bonds on the molecules such as proteins 3. Which condition tested in lab denatured the catalase? 4. Why does product production slow down over time, even at optimal pH, temperature and enzyme concentration? 5. Amylase is an enzyme that is produced in human salivary glands and secreted into the mouth to aid in...
enzyme concentration decreases. Amylase is an enzyme that is produced in human salivary glands and secreted...
enzyme concentration decreases. Amylase is an enzyme that is produced in human salivary glands and secreted into the mouth to aid in starch digestion. a. What is the substrate in this reaction? b. Where on the enzyme will the substrate bind? c. Based on the fact that this enzyme is found in our bodies, what do you hypothes would be the: optimal temperature for amylase? optimal pH for amylase? d. What do you think happens to the amylase when it...
ruin the following table to describe the effect incubating an organism above or below its optimal...
ruin the following table to describe the effect incubating an organism above or below its optimal growth temperature has on molecules required for survival? Structure Below the optimal Temperature Above the optimal temperature Enzymes Membranes 5. Design an experiment which would allow you to determine whether an organism isolated at room temperature (around 20 °C) was a psychrotroph or a mesophile. Make sure your answer predicts where you would expect to see growth for each type of organism, and how...
2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
1. What would be some of the possible positive and negative controls of the effect of...
1. What would be some of the possible positive and negative controls of the effect of pH in potato enzyme lab? 2. What would be the positive and negative controls of the effect on temperature in a potato enzyme lab experiment? 3. Positive and negative controls on the effect of salt in potato enzyme lab?
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a pr...
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a protein? -CH-CH3 он CH2-CH-CH3 он CH3 11) What process occurs when heat, acids, bases, and heavy metal ions cause loss of biological function of a protein? 12) Answer the following questions regarding enzyme activity: a) The optimum temperature and pH for most biological enzymes is b) The molecules to which an enzyme binds are called the c) The...
3. You did some research after the Enzyme lab and you found out that the optimal...
3. You did some research after the Enzyme lab and you found out that the optimal activity for the cellobiase enzyme was 40°C. In class, you conducted your experiment at room temperature, which is about 25°C. What would have happened to your experiment if you conducted it experiment inside a room at 5°C? What about if you conducted inside a room at 100°C? Hint: Tell me what will happen to your enzyme in each scenario AND if you expect to...
What would happen if BsteII-HF had an optimal temperature of 30°C but we used the same...
What would happen if BsteII-HF
had an optimal temperature of 30°C but we used the same double
digestion protocol as we used in lab 4? (2pts)
Note: consider all the cloning steps for this
question
Double digestion of the the CAN1.Z ORNA and the PMD2 plasmid Bola I-HF SCHI CANL.Z! CANL.Z -SHI ©- MD2 CANLY → PMD2 CANLLY Bok I-HF You will cut both the plasmid PMD2 and the CAN1.Z ORNA using 2 enzymes (Bstell-HF and Sphl). These are two...
Describe the function of how internal controls reduce risk and how would one design controls to...
Describe the function of how internal controls reduce risk and how would one design controls to reduce risk?
Enzymes Temperature is an important factor that can regulate enzyme-mediated functions. Temperature is one of the tightly homeostatically controlled variables in the human body. What are the symptoms of loss of temperature control during a high fever? Explain why these symptoms occur based on enzyme function and cell signaling effects that are dependent on temperature. Based on Figure 1, explain how the temperature optimum of the activity of enzymes is expected to differ in organisms depending on where they live....
POSTLAB QUESTIONS Name: Masa Ahmad 1. What were the optimal pH, temperature and enzyme concentration for catalase? 2. Define the term denatured. Denatured is the Breaking the bonds on the molecules such as proteins 3. Which condition tested in lab denatured the catalase? 4. Why does product production slow down over time, even at optimal pH, temperature and enzyme concentration? 5. Amylase is an enzyme that is produced in human salivary glands and secreted into the mouth to aid in...
enzyme concentration decreases. Amylase is an enzyme that is produced in human salivary glands and secreted into the mouth to aid in starch digestion. a. What is the substrate in this reaction? b. Where on the enzyme will the substrate bind? c. Based on the fact that this enzyme is found in our bodies, what do you hypothes would be the: optimal temperature for amylase? optimal pH for amylase? d. What do you think happens to the amylase when it...
ruin the following table to describe the effect incubating an organism above or below its optimal growth temperature has on molecules required for survival? Structure Below the optimal Temperature Above the optimal temperature Enzymes Membranes 5. Design an experiment which would allow you to determine whether an organism isolated at room temperature (around 20 °C) was a psychrotroph or a mesophile. Make sure your answer predicts where you would expect to see growth for each type of organism, and how...
2. hypothesize the best conditions (pH and temperature) under
which the enzyme chymotrypsin functions with an appropriate
reference. also, hypothesize what will occur in the presence of an
inhibitor and the type of inhibition.
EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...
10) Would an amino acid with the given side chain be most likely found in the hydrophobic or hydrophilic region of a protein? -CH-CH3 он CH2-CH-CH3 он CH3 11) What process occurs when heat, acids, bases, and heavy metal ions cause loss of biological function of a protein? 12) Answer the following questions regarding enzyme activity: a) The optimum temperature and pH for most biological enzymes is b) The molecules to which an enzyme binds are called the c) The...
3. You did some research after the Enzyme lab and you found out that the optimal activity for the cellobiase enzyme was 40°C. In class, you conducted your experiment at room temperature, which is about 25°C. What would have happened to your experiment if you conducted it experiment inside a room at 5°C? What about if you conducted inside a room at 100°C? Hint: Tell me what will happen to your enzyme in each scenario AND if you expect to...
What would happen if BsteII-HF
had an optimal temperature of 30°C but we used the same double
digestion protocol as we used in lab 4? (2pts)
Note: consider all the cloning steps for this
question
Double digestion of the the CAN1.Z ORNA and the PMD2 plasmid Bola I-HF SCHI CANL.Z! CANL.Z -SHI ©- MD2 CANLY → PMD2 CANLLY Bok I-HF You will cut both the plasmid PMD2 and the CAN1.Z ORNA using 2 enzymes (Bstell-HF and Sphl). These are two...
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