Question

5. The following data were obtained when Bio 106 students created solutions of the following concentrations (column 1), These students then placed three different samples of each concentration in a spectrophotometer and measured the absorbance. The following data table was created by these students. Create a standard curve from the data in the following table. Be sure to calculate the average absorbance for each concentration (round to two decimal places). After you have created the table, answer the following questions. d Concentration Average Absorbance # 3 Absorbance #1 Absorbance #2 (4g/mL) Absorbance 0.18 0.22 0.23 3 0.38 0.40 0.39 0.59 0.67 0.60 0.69 6 0.70 0.65 0.88 0.87 0.87 1.10 10 1.08 1.12 What are the independent and dependent variable(s) in the above table? a. b. What would the absorbance be of a solution with a concentration of 4 ug/mL? Which concentration had the most precise/accurate absorbance measurement? How did you determine this? c. d. Now, suppose you had an unknown sample that had been diluted as follows: 1 mL of unknown + 4 mL of solvent. This diluted sample was then tested and had an absorbance of 0.7. What is the concentration of the original sample? SHOW YOUR WORK! Suppose you had a solution with an absorbance of 2. Could you determine its concentration using the standard curve? Explain why or why not. e.

Answer 1

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