Suppose your professor handed you a test tube with 2.0 ml of an E. Coli broth culture in it and...
Homework Answers
Dilute the 2.0 mL bacterial culture with 18.0 mL sterile broth culture to give a total of 20.0 mL.
Therefore, 2.0 mL / 20.0 mL = 10, hence the 10 fold dilution.
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Suppose your professor handed you a test tube with 2.0 ml of an E. Coli broth culture in it and...
suppose your professor handed you a test tube with 2.0 mL of broth culture in it and told you to...
suppose your professor handed you a test tube with 2.0 mL of broth culture in it and told you to make a 10^-1 dilution of entire culture, how?
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
14. You inoculated a 1 mL tube of sterile LB broth with E. coli, and let...
14. You inoculated a 1 mL tube of sterile LB broth with E. coli, and let it grow @37C overnight. The next day you extracted a total of 2.15 ug of DNA from this culture. Calculate how many cells were in that 1 mL of culture? (Hint: Use the value derived above to do this calculation). 5 points 15. If you assume that your 2.15 ug of DNA isolated from E. coli had an average length of 10,000 bp, approximately...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
may you help me figure out how to solve 7-9 Standard plate count. Include formulas and...
may you help me figure out how to solve 7-9
Standard plate count. Include formulas and calculations. Scientific notation. Show your work. UNITSI 7. Suppose you have 2.0 ml of an undiluted E.coli broth and need to begin your serial dilution by making a 10. solution. How much diluent would you need if you wanted to use the entire 2 ml of E. coli broth? 110-ml 102 = 2mL ol os dition 10 8. If you transfer 0.1 ml of...
You want to dilute and plate an E. coli culture that has 3 *10 8 cells/ml....
You want to dilute and plate an E. coli culture that has 3 *10 8 cells/ml. If you needed to dilute the above culture, starting from the undiluted stock, to a concentration of 500 cells/ml, how could you perform a serial dilution to achieve the desired final concentration?
You inoculate a strain of E. coli into a broth culture and allow it to grow....
You inoculate a strain of E. coli into a broth culture and allow it to grow. The broth medium contains three sugars: glucose, lactose, and maltose. The strain you are growing prefers lactose to maltose. You measured the growth by doing a viable cell count and drew a growth curve after calculating the number of CFU/ml over time. There was an initial lag phase, followed by an exponential phase. There was a second lag phase of approximately 30 minutes followed...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect...
You have a stock of an E. coli cell culture of an unknown concentration. You want...
You have a stock of an E. coli cell culture of an unknown concentration. You want to set up a serial dilution experiment so that you can count the viable colonies and determine the concentration of the stock in cells/ml. In tube A, you add 1 ml of the stock to 999 ml of media. In tube B, you add 1 ml of the material from tube A to 24 ml of media. In tube C, you add 50 microliters...
You inoculate a strain of E. coli into a broth culture and allow it to grow....
You
inoculate a strain of E. coli into a broth culture and allow it to
grow. The broth medium contains three sugars: glucose, lactose, and
maltose. The strain you are growing prefers lactose to maltose. You
measured the growth by doing a viable cell count and drew a growth
curve after calculating the number of CFU/ml over time. There was
an initial lag phase, followed by an exponential phase. There was a
second lag phase of approximately 30 minutes followed...
14. You inoculated a 1 mL tube of sterile LB broth with E. coli, and let it grow @37C overnight. The next day you extracted a total of 2.15 ug of DNA from this culture. Calculate how many cells were in that 1 mL of culture? (Hint: Use the value derived above to do this calculation). 5 points 15. If you assume that your 2.15 ug of DNA isolated from E. coli had an average length of 10,000 bp, approximately...
may you help me figure out how to solve 7-9
Standard plate count. Include formulas and calculations. Scientific notation. Show your work. UNITSI 7. Suppose you have 2.0 ml of an undiluted E.coli broth and need to begin your serial dilution by making a 10. solution. How much diluent would you need if you wanted to use the entire 2 ml of E. coli broth? 110-ml 102 = 2mL ol os dition 10 8. If you transfer 0.1 ml of...
You inoculate a strain of E. coli into a broth culture and allow it to grow. The broth medium contains three sugars: glucose, lactose, and maltose. The strain you are growing prefers lactose to maltose. You measured the growth by doing a viable cell count and drew a growth curve after calculating the number of CFU/ml over time. There was an initial lag phase, followed by an exponential phase. There was a second lag phase of approximately 30 minutes followed...
You
inoculate a strain of E. coli into a broth culture and allow it to
grow. The broth medium contains three sugars: glucose, lactose, and
maltose. The strain you are growing prefers lactose to maltose. You
measured the growth by doing a viable cell count and drew a growth
curve after calculating the number of CFU/ml over time. There was
an initial lag phase, followed by an exponential phase. There was a
second lag phase of approximately 30 minutes followed...
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