Design a vector polylinker for directional cloning
A vector has a polylinker containing restriction sites in the following order:HindIII, SacI, XhoI, BglII, XbaI, and ClaI.
a. Give a possible nucleotide sequence for the polylinker.
b. The vector is digested withHindIII and ClaI. A DNA segment contains a HindIII restriction site fragment 650 bases upstream from a ClaI site. This DNA fragment is digested with HindIII and ClaI, and the resulting HindIII-ClaI fragment is directionally cloned into the HindIII-ClaI digested vector. Give the nucleotide sequence at each end of the vector and the insert and show that the insert can be cloned into the vector in only one orientation.
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