A polylinker for expression of aβ-galactosidase fusion protein
Figure shows a polylinker that falls within theβ-galactosidase coding region of the lacZ gene. This polylinker serves as a cloning site in a fusion protein expression vector where the cloned insert is expressed as a β-galactosidase fusion protein. Assume the vector polylinker was cleaved withBamHI and then ligated with an insert whose sequence reads
GATCCATTTATCCACCGGAGAGCTGGTATCCCCAAAAGACGGCC...
What is the amino acid sequence of the fusion protein? Where is the junction betweenβ- galactosidase and the sequence encoded by the insert? (Consult the genetic code table on the inside front cover to decipher the amino acid sequence.)
We need at least 10 more requests to produce the solution.
0 / 10 have requested this problem solution
The more requests, the faster the answer.