Overlapping restriction sites in a plasmid vector polylinker
The nucleotide sequence of a polylinker in a particular plasmid vector is
-GAATTCCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCThis
polylinker contains restriction sites forBamHI, EcoRI, PstI, SalI, SmaI, SphI, and XbaI. Indicate the location of each restriction site in this sequence. (See Table 10.2 of restriction enzymes for their cleavage sites.)
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