Question

1. The protein's primary structure. You can get this from any of several databases. I usually go to Uniprot (see the example) and search using the protein's name. Once you get a list of the various proteins that share this name, pick the appropriate human version and click on its database identifier. 2. An estimate of its isoelectric point (pl) and molecular weight (MW). Indicate if it should be an anion. a cation, or a neutral compound (zwitterion) at neutral pH (7.0). I use the program at expasy - just copy the primary structure (numbers and all), paste it into the box and click the button. The URL is in your class notes. 3. Describe the protein's secondary and tertiary structures. Uniprot gives you the secondary structures by displaying the protein sequence as a line and coloring the line different colors corresponding to a-helixes, B-strands, and turns, random coil is usually gray. Use the snipping tool in Windows) or suitable software to take a picture and insert it into your file. The tertiary structure is present as a cartoon that can be moved using your mouse. Move it around until you like what you see and take a picture. Paste the picture into your file. Does the protein have a cofactor or prosthetic group? 4. Describe the protein's natural function. You may use any source, but please use your own words instead of copying and pasting from your source. If it is an enzyme, you can copy a picture that shows the reaction it catalyzes and write a few words about how that reaction fits into the big picture. 5. How is this protein used as a biomarker?
need help pickimg an list of proteins that are used as biomakers and answering these questions about it

Answer 1

structure 2) protein's I. OJ - e - Ć - i - N my IZ H 1 2) compound (zwiter A protein is a neutral Cion) at neutral pH A I-J- - - c- o NH, =

Secondary structure & Wor H ß- pleated to Nut it to HAT H Sheet M wc-o -c-on-4 0 - Tertiary structure. w- a-helix DON
proteins are used as hormones both endocrine and exocrine , they are used as enzyme for speeding up the reaction mechanism of our body metabolism . For example hydrolysis of glucose in presence of zymase into ethyl alcohol and carbon dioxide and several other .

5. A biomarker is a substance that is used in biology to mark or isolate certain tissues

Some of the examples of biomarker protein are

Adiponectin , alpha -feto protein , protein Oct-4 .