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4. For questions 1-6 in lab manual (page 73): First write down each question and then give the corresponding answers. 4.1. Da
4. 5. Give the formula for determining bacterial counts 4. 6. (Show calculation steps). Give the bacterial count per millilit
4. For questions 1-6 in lab manual (page 73): First write down each question and then give the corresponding answers. 4.1. Data collection: #of bacteria (Y) 1 x 10 9 0.5 x 10 9 0.25 x 10A9 CFU/ML 0.042 x 10 9 CFU/ML 0.020 x 10 9 CFU/ML Absorbance Dilutions CFU/ML original E. coli 0.674 CFU/ML 0.217 ½2 0.179 /4 0.084 1/8 0.040 1/16
4. 5. Give the formula for determining bacterial counts 4. 6. (Show calculation steps). Give the bacterial count per milliliter of E. coli suspension in the original culture tube. 4. 7. Finally, using Excel graphic software, plot a standard curve using data from step 4.1. When entering your data in Excel graphic, start with the zero values (X-axis-0 and Y-axis-0). Use absorbance column values for X- axis and the number of E. coli calculated for Y-axis. To plot the graph, follow the steps given in your manual (pages 76-78). I
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Answer #1

0.F Diluln Fachw 4 No badena x D-F CFUJm 6-5x10 2-1x1Dl 0.25 x041X 6.042 x 0.336x107 Dihlon=2 CFun DiohnV4 2 . CFulm 3. CR CF

1.2 0.8 0.6 0.4 0 2 0.4 O 5 0.6 0.7 0.1 0.2 0.3 0 8 OD Number of bacteria X 10A9

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