Question

Endospore stain positive or negative

Endospore stain postive or negative

First picture is one slide, other two pictures are from the same slide

Answer 1

Answer and explaination -

Vegetative cells are subjected to stressed environmental conditions like nutrition deficiency then they produces though dormant and inactive form called as endospore.

They are resistant to staining also. so they are stained with Schaefer Fulton method, to distinguish between vegetative cells and endospores. After staining the vegetative cells appears as red or pink, background looks colourless and the endospores are green in colour.

In the given images 1st and 2nd image show's only pink coloured vegetative cells. There is no endospores present.

In 2nd image of 2nd slide shows little green colour but is due to the remain malechite green colour after incomplete washing.

Therefore given both the slides are endospores negative.ony vegetative cells are present.