Question

V-type proton ATPase (proton pump):

a: What type of transport is a V-type proton ATPase (an integral membrane protein) uses ATP directly to transport protons across the membrane ? (PRIMARY OR SECONDARY)

b: With the understanding of sodium-potassium pump, describe how a proton pump would use ATP to move protons against their concentration gradient (out of the cell) ? please do not say energy.

Answer 1

a: What type of transport is a V-type proton ATPase (an integral membrane protein) uses ATP directly to transport protons across the membrane ? (PRIMARY OR SECONDARY)

In this case, V-type proton ATPase is a primary active transport. In the primary active transport, the force used to transport molecules is derived directly from the breakdown of ATP. For the contrary, a secondary active transport uses a source of force derived from a secondarily force stored in form of ionic concentration differences between the two sides of the membrane. In the case of V-type proton ATPase, Its main function is to acidify intracellular organelles and pump protons across the plasma membranes using the ATP hydrolysis.