pleas help me answer this question
Homework Answers
The image in question is hard to read. Please submit the question at least in a readable format.
Answer:
The DNA electrophoresis pattern confirms that the DNA has been cut into two bands. Thus, there is one cut site. The uncut DNA is between 5 and 6 kb, while on being cut, one band is 3 kb and the other between 2 and 3 kb. Thus, the right option has to be the one with a single cut site.
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pleas help me answer this question
the
line are the cut sites and based off of...
Hello, I came across this question and I am confused on where to start. Restriction mapping...
Hello,
I came across this question and I am confused on where to
start.
Restriction mapping of a linear piece of DNA reveals the following EcoRI restriction sites. Site 1 Site 2 _2kb 4kb 5kb 1. This piece of DNA is cut by EcoRI the resulting fragments are separated by gel electrophoresis, and the gel is stained. Draw a picture of the bands that will appear in the gel below 2.If a mutation that alters EcoRI site 1 occurs in...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5 > A B C D E Restriction mapping of a linear piece of DNA reveals the EcoRI restriction sites. EcoRI site ! EcoRI site 2 2 kb. 4 kb 5 kb 11 kb 6 kb 5 kb 4 kb --3.5 kb 2 kb In each of the scenarios described, DNA is cut by EcoRI and the resulting fragments are separated by gel electrophoresis. The...
Can someone help me with this homework question on gel electrophoresis? Translocation and deletion 3. You...
Can someone help me with this homework question on gel
electrophoresis?
Translocation and deletion 3. You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in determining whether a patient has a mutation within a specific DNA region. You know that this DNA segment of the "normal" gene does not include an EcoRI restriction site; but the mutated DNA segment of the same gene contains an EcoRI restriction site due to a point mutation at...
Can someone please help me answer these? Neat work is greatly appreciated. Explanation will also be...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
Hi can someone help me understand part C and why the drawn in red lines are...
Hi can someone help me understand part C and why the
drawn in red lines are where they are.
Basically from the bp given how can I go back to cm so I can
drawn them into the picture provided?
Do not need help with A or B.
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
Ive added an image at the bottom of the question with my attempt based on my...
Ive added an image at the bottom of the question with my attempt
based on my understanding of the question. can someone please tell
me if i have understood the question correctly and if not please
explain to me what it actually wants and how to go about completing
it.
A plasmid (pUSQ2) is cut with a series of four restriction
enzymes (A, B, C and D) and this produces the following bands after
submarine gel electrophoresis:
Enzyme(s)...
ok so this is a practice problem and I just want to make sure its correct....
ok so this is a practice problem and I just want to make sure
its correct. the first pic is the whole question the the second is
the pic in the second part of the question. the third pic is the
same pic in the first and second part of this question I just
wanted to make sure the picturs are big enough to see thats why I
uploaded them. If you have a problem please let me know.
Design...
So this question is asking for me to determine what genotype these individuals are based off...
So this question is asking for me to determine what genotype
these individuals are based off the gel electrophoresis scan next
to it.
5. Observe the photograph of a stained gel containing the PMCT118 amplified results from 6 unknown people. a. Lanes 1, 4, 7 and 10 contain and allelic ladder that corresponds to a sample mixture containing all 32 alleles at the PMCT118 locus 1 2 3 4 5 6 7 8 9 10 (alleles 13, 20, 30 and...
Hello,
I came across this question and I am confused on where to
start.
Restriction mapping of a linear piece of DNA reveals the following EcoRI restriction sites. Site 1 Site 2 _2kb 4kb 5kb 1. This piece of DNA is cut by EcoRI the resulting fragments are separated by gel electrophoresis, and the gel is stained. Draw a picture of the bands that will appear in the gel below 2.If a mutation that alters EcoRI site 1 occurs in...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5 > A B C D E Restriction mapping of a linear piece of DNA reveals the EcoRI restriction sites. EcoRI site ! EcoRI site 2 2 kb. 4 kb 5 kb 11 kb 6 kb 5 kb 4 kb --3.5 kb 2 kb In each of the scenarios described, DNA is cut by EcoRI and the resulting fragments are separated by gel electrophoresis. The...
Can someone help me with this homework question on gel
electrophoresis?
Translocation and deletion 3. You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in determining whether a patient has a mutation within a specific DNA region. You know that this DNA segment of the "normal" gene does not include an EcoRI restriction site; but the mutated DNA segment of the same gene contains an EcoRI restriction site due to a point mutation at...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
Hi can someone help me understand part C and why the
drawn in red lines are where they are.
Basically from the bp given how can I go back to cm so I can
drawn them into the picture provided?
Do not need help with A or B.
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes...
Ive added an image at the bottom of the question with my attempt
based on my understanding of the question. can someone please tell
me if i have understood the question correctly and if not please
explain to me what it actually wants and how to go about completing
it.
A plasmid (pUSQ2) is cut with a series of four restriction
enzymes (A, B, C and D) and this produces the following bands after
submarine gel electrophoresis:
Enzyme(s)...
ok so this is a practice problem and I just want to make sure
its correct. the first pic is the whole question the the second is
the pic in the second part of the question. the third pic is the
same pic in the first and second part of this question I just
wanted to make sure the picturs are big enough to see thats why I
uploaded them. If you have a problem please let me know.
Design...
So this question is asking for me to determine what genotype
these individuals are based off the gel electrophoresis scan next
to it.
5. Observe the photograph of a stained gel containing the PMCT118 amplified results from 6 unknown people. a. Lanes 1, 4, 7 and 10 contain and allelic ladder that corresponds to a sample mixture containing all 32 alleles at the PMCT118 locus 1 2 3 4 5 6 7 8 9 10 (alleles 13, 20, 30 and...
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