How do you quantitate expression of a single gene?
For a hypothetical gene, how do the terms dominance and recessive relate to gene expression and how genes work? Question 22 options: a) A dominant phenotype results in the expression of the most beneficial genes resulting in increased chances of survival of the organism b) The allele with the dominant phenotype inhibits transcription of the allele for the recessive phenotype c) Both alleles get expressed, but the product of the allele with the dominant phenotype masks the phenotype of the...
If you want to measure gene expression using PCR, why do you have to use cDNA and not gDNA?
You hypothesize that expression of Gene X, a gene for which you know the complete sequence in the organism of interest, may be an important contributor to the development of a phenotype (trait) that you study (e.g. eye development). What approach(es) would you use to manipulate the expression pattern of the gene to test your hypothesis?
Discuss mechanisms by which gene expression may be altered. How do these alterations induce cancer-causing mutations in cell DNA? Explain how cancer is formed.
How do transcription factors affect gene expression? a.) They are molecules that can turn genes on or off. b.) They are mRNAs that contain extra information that is not part of the DNA genome. c.)They modify the chromosome structure to create mutations. d.) They are signals that tell cells whether to divide or not.
2) What is the molecular reason that loss of expression of a single Hox gene due to a mutation typically transforms the segment (or group of segments) into an anterior segmental fate? 3) Discuss the concept of a "molecular address" in the Drosophila cellular blastoderm stage embryo. Be sure to discuss both which genes are involved in setting up the address and which are involved in interpreting the address. 4) Explain the reason (at the level of molecules and regulatory...
This is the gene expression analysis of actin and EGFR in breast cancer What do the images represent? Describe axis. What can you tell about the importance of actin and EGFR genes in breast cancer?
Define constitutive gene expression and differential gene expression.
How might you use molecular cloning and heterologous expression to identify a gene or genes responsible for a particular phenotype (e.g. pectin degradation)? What are some advantages and disadvantages?
What do we call the phenomenon where the expression of one gene or two genes can then affect the expression of another gene? Why is this concept so powerful in terms of genetic expression in multicellular organisms?